Inhibitory Immune Checkpoint panel 1 - Human IHC (ab278174)
Overview
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Product name
Inhibitory Immune Checkpoint panel 1 - Human IHC -
Species reactivity
Reacts with: Human -
Product overview
Inhibitory Immune Checkpoint panel 1 - Human IHC ab278174 contains multiple trial-sized versions of anti-human antibody clones against PD-L1, PD1, LAG3, TIM 3, VISTA, CTLA4 and TIGIT specifically selected for high performance in various applications. They are provided as a sampler panel to allow you to easily evaluate each antibody.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [73-10] to PD-L1 (20 μL) ab228415
- Rabbit monoclonal [EPR4877(2)] to PD1 (20 μL) ab137132
- Rabbit monoclonal [EPR20261] to LAG-3 (20 μL) ab209236
- Rabbit monoclonal [EPR22241] to TIM 3 (20 μL) ab241332
- Rabbit monoclonal [EPR21050] to VISTA (20 μL) ab230950
- Rabbit monoclonal [CAL49] to CTLA4 (20 μL) ab237712
- Rabbit monoclonal [BLR047F] to TIGIT - BSA free (20 μL) ab243903
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis. Please refer to the ‘Associated products’ section below.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Please refer to the ‘Associated products’ section below.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit ab237712 - Anti-CTLA4 antibody [CAL49] 2 x 10µl ab209236 - Anti-Lymphocyte Activation Gene 3 antibody [EPR20261] 2 x 10µl ab137132 - Anti-PD1 antibody [EPR4877(2)] 2 x 10µl ab228415 - Anti-PD-L1 antibody [73-10] 2 x 10µl ab243903 - Anti-TIGIT antibody [BLR047F] 2 x 10µl ab241332 - Anti-TIM 3 antibody [EPR22241] 2 x 10µl ab230950 - Anti-VISTA antibody [EPR21050] 2 x 10µl -
Research areas
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Cellular localization
CTLA4: Cell membrane. Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation and. TIM 3: Membrane. Cell junction. Localizes to the immunological synapse between CD8+ T-cells and target cells. PD1: Membrane. LAG-3: Membrane. PD-L1: Cell membrane and Endomembrane system. VISTA: Membrane. TIGIT: Cell membrane. -
Database links
- Entrez Gene: 1493 Human
- Entrez Gene: 201633 Human
- Entrez Gene: 29126 Human
- Entrez Gene: 3902 Human
- Entrez Gene: 5133 Human
- Entrez Gene: 64115 Human
- Entrez Gene: 84868 Human
- Omim: 123890 Human
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Formalin-fixed, paraffin-embedded human tonsil tissue stained for TIGIT using ab243903 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CTLA4 with ab237712 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human tonsil is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.
The section was incubated with ab237712 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CTLA4 using ab237712 at 0.25 μg/ml in immunohistochemical analysis.
Incubate with primary antibody for 75 minutes at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Formalin-fixed, paraffin-embedded human lymph node tissue stained for CTLA4 using ab237712 at 0.25 μg/ml in immunohistochemical analysis.
Incubate with primary antibody for 75 minutes at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling VISTA with ab230950 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Membranous and cytoplasmic staining in tumor-in?ltrating leukocytes of human lung cancer (PMID: 28236118, PMID: 28507801) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling VISTA with ab230950 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Membranous and cytoplasmic staining in leukocytes of human spleen (PMID: 21383057, PMID: 24691993) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemical analysis of paraffin-embedded human liver tissue labeling TIM 3 with ab241332 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and membranous staining on Kupffer cells of human liver (PMID: 27192565) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling TIM 3 with ab241332 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and membranous staining on both infiltrated immunocytes and tumor cells of human lung cancer (PMID: 22586058) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemical analysis of paraffin-embedded human tonsil Hodgkin’s lymphoma labeling LAG-3 with ab209236 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on immunocytes of the human Hodgkin’s lymphoma [PMID: 11527700; PMID: 16757686].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LAG-3 with ab209236 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic cytoplasmic staining on immunocytes of human tonsil [PMID: 11527700; PMID: 16757686].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling PD1 with purified ab137132 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control: Secondary antibody only using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling PD1 with ab137132 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA antigen retrieval solution, and microwave treatment for 20 min at 20% power. Anti-Rabbit HRP polymer was used as secondary antibody. Opal tyramide amplification was performed using Opal 540 fluorophore. Counterstained with DAPI stain.
Image scanned with Vectra 3.0 and analyzed via Inform software.
This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human T-cell lymphoma tissue labeling PD1 with unpurified ab137132 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling PD1 with unpurified ab137132 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human placenta staining PD-L1 with ab228415 at 1/500 dilution. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10 mins. The section was incubated with ab228415 for 10 mins at room temperature. The secondary antibody used was ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained Hematoxylin. Performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human lung carcinoma staining PD-L1 with ab228415 at 1/500 dilution. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10 mins. The section was incubated with ab228415 for 10 mins at room temperature. The secondary antibody used was ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained Hematoxylin. Performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling PD-L1 with ab228415 at 1/5000 dilution. The tissue was incubated with ab228415 at 4? overnight. followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Membranous and cytoplasmic staining in human placenta (PMID: 12538684) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Antigen retrieval: Universal HIER antigen retrieval reagent (10X) (ab208572).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Inhibitory immune checkpoint panel 1 - human IHC (ab278174)IHC image of ab228415 staining PD-L1 in human tonsil formalin fixed paraffin embedded tissue sections*, performed on a Leica BOND RX (standard Protocol F, Polymer Refine kit).
The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 30 mins at 98°C. The section was then incubated with ab228415, 0.06μg/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system for 8 minutes at room temperature. DAB was used as the chromogen for 10 minutes at room temperature. The section was then counterstained with hematoxylin, blued, dehydrated, cleared and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
