ATPase Assay Kit (ab270551)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
Overview
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Product name
ATPase Assay Kit -
Detection method
Colorimetric -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
ATPase Assay Kit (ab270551) employs a 96-well plate format with all of the reagents necessary for measuring ATPase activity. The kit also contains PiColorLock™ (a superior malachite green reagent) which has special additives to prevent backgrounds arising from non-enzymatic ATP hydrolysis. The PiColorLock™ assay is based on the change in absorbance of the dye malachite green in the presence of phosphomolybdate complexes. Unlike most malachite dye-based solutions, PiColorLock™ gives a stable end-point signal and is not prone to precipitation. PiColorLock™ has special additives to prevent backgrounds arising from non-enzymatic ATP hydrolysis.
*For samples with free Pi, please check the Inorganic Phosphate Binding Resin (ab270547).
This product is manufactured by Expedeon, an Abcam company. It was previously called ATPase Enzyme Assay Kit. Expedeon product code 601-0120 is the same as the 2 x 96 tests size of this product.
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Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 2 x 96 tests ab274109 - 0.1 M Magnesium chloride 1 x 1500µl ab273991 - 0.1 mM Pi Standard 1 x 5ml ab274110 - 0.5 M Tris pH 7.5 1 x 5ml ab274107 - 96-well clear low binding plates 1 unit ab273989 - Accelerator 1 x 500µl ab274108 - ATP (lyophilized) 4 vials ab274293 - PiColorLock 1 x 10ml ab273990 - Stabilizer 1 x 5ml
Images
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Standard curve
Data provided for demonstration purposes only.
Standard Curve for ATPase Assay Kit (ab270551).
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Background stabilityATP has been incubated in three detection reagents. A steadily rising background signal is seen with competitor reagents, whereas PiColorLock™ provides baseline readings.
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Dynamic rangeDynamic Range for ATPase Assay Kit (ab270551).
PiColorLockTM has been designed to have a large linear range, thus reducing the need for sample dilution. Competitors’ products are linear over a much narrower range of concentrations.