Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UBA5 antibody [EPR11729] (ab177478)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11729] to UBA5
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-UBA5 antibody [EPR11729]
    See all UBA5 primary antibodies

  • Description

    Rabbit monoclonal [EPR11729] to UBA5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human UBA5 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9GZZ9

  • Positive control

    • Caco-2, Jurkat and 293T cell lysate: Human prostate gastric carcinoma and Human prostate tissues; Caco-2 cells; Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11729

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-UBA5 antibody [EPR11729] (ab177478)
    Western blot - Anti-UBA5 antibody [EPR11729] (ab177478)
    All lanes : Anti-UBA5 antibody [EPR11729] (ab177478) at 1/1000 dilution

    Lane 1 : Caco-2 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 45 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-UBA5 antibody [EPR11729] (ab177478)
    Immunocytochemistry/ Immunofluorescence - Anti-UBA5 antibody [EPR11729] (ab177478)

    Immunofluorescent analysis of Caco-2 cells labeling UBA5 with ab177478 at 1/50 (green) and DAPI staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA5 antibody [EPR11729] (ab177478)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA5 antibody [EPR11729] (ab177478)

    Immunohistochemical analysis of paraffin embedded Human prostate gastric carcinoma tissue labeling UBA5 with ab177478 at 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA5 antibody [EPR11729] (ab177478)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA5 antibody [EPR11729] (ab177478)

    Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling UBA5 with ab177478 at 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-UBA5 antibody [EPR11729] (ab177478)
    Flow Cytometry - Anti-UBA5 antibody [EPR11729] (ab177478)

    Flow Cytometrical analysis of permeabilized Jurkat cells labeling UBA5 with ab177478 at 1/10 (red) or a rabbit IgG (negative) (green).

  • Anti-UBA5 antibody [EPR11729] (ab177478)
    Anti-UBA5 antibody [EPR11729] (ab177478)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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