Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
Key features and details
- Rabbit polyclonal to Tyrosine Hydroxylase - Neuronal Marker
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Tyrosine Hydroxylase antibody - Neuronal Marker
See all Tyrosine Hydroxylase primary antibodies -
Description
Rabbit polyclonal to Tyrosine Hydroxylase - Neuronal Marker -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatWB Rat
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Immunogen
Full length SDS denatured protein (purified from pheochromocytoma) (Rat).
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General notes
ab112 can be used as a marker for dopaminergic and noradrenergic neurons.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 0.01% BSA, 0.87% Sodium chloride, 50% Glycerol, 0.238% HEPES -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Predicted band size: 60 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)Paraffin embedded sections of rat brain tissue were stained for Tyrosine Hydroxylase with ab112 at 1/5000 dilution in immunohistochemical analysis (Panel 1).
Panel 2 shows an image in which ab112 was replaced with a Rabbit IgG1 isotype control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) This image is courtesy of an Abreview submitted by Dr Guillermo Estivill-Torrusab112 at 1/500 staining rat brain tissue (ab29475) sections by IHC-P.
The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 14 hours. A biotinylated swine anti-rabbit IgG was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) This image is courtesy of an anonymous Abreviewab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry.
The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.
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Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + rat caudate lysate at 10 µg
Predicted band size: 60 kDa
