Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7102] to Monoamine Oxidase B/MAOB
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-Monoamine Oxidase B/MAOB antibody [EPR7102]
See all Monoamine Oxidase B/MAOB primary antibodies
Description

Rabbit monoclonal [EPR7102] to Monoamine Oxidase B/MAOB
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Monoamine Oxidase B/MAOB aa 1-100. The exact sequence is proprietary.
Positive control
- Human fetal liver, fetal kidney, platelet, fetal brain and SH SY5Y lysates; Human brain and kidney tissues.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product was previously labelled as Monoamine Oxidase B

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.90 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7102
Isotype

IgG
Research areas

Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg

Secondary
Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 59 kDa
Observed band size: 59 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/2000 dilution (purified)

Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 59 kDa
Observed band size: 59 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

Immunohistochemical staining of paraffin embedded human liver with purified ab133270 at a working dilution of 1/300. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/1000 dilution (Unpurified)

Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human platelet lysate
Lane 4 : Human fetal brain lysate
Lane 5 : SH SY5Y cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

Immunohistochemical analysis of paraffin embedded human brain tissue labelling Monoamine Oxidase B/MAOB with unpurified ab133270 at 1/100Produced under U.S. Patent No. 5,675,063.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

Immunohistochemical analysis of paraffin embedded human kidney tissue labelling Monoamine Oxidase B/MAOB with unpurified ab133270 at 1/100 dilution.
OI-RD Scanning - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com