Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7102] to Monoamine Oxidase B/MAOB
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Monoamine Oxidase B/MAOB antibody [EPR7102]
    See all Monoamine Oxidase B/MAOB primary antibodies

  • Description

    Rabbit monoclonal [EPR7102] to Monoamine Oxidase B/MAOB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Monoamine Oxidase B/MAOB aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Human fetal liver, fetal kidney, platelet, fetal brain and SH SY5Y lysates; Human brain and kidney tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Monoamine Oxidase B

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
    Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
    Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg

    Secondary
    Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
    Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
    All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/2000 dilution (purified)

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
    Immunohistochemical staining of paraffin embedded human liver with purified ab133270 at a working dilution of 1/300. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
    Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
    All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/1000 dilution (Unpurified)

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human platelet lysate
    Lane 4 : Human fetal brain lysate
    Lane 5 : SH SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 59 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

    Immunohistochemical analysis of paraffin embedded human brain tissue labelling Monoamine Oxidase B/MAOB with unpurified ab133270 at 1/100Produced under U.S. Patent No. 5,675,063.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

    Immunohistochemical analysis of paraffin embedded human kidney tissue labelling Monoamine Oxidase B/MAOB with unpurified ab133270 at 1/100 dilution.

  • OI-RD Scanning - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
    OI-RD Scanning - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)
    Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270)

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