Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1062Y] to Tuberin (phospho S939) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free
    See all Tuberin primary antibodies

  • Description

    Rabbit monoclonal [EP1062Y] to Tuberin (phospho S939) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247344 is the carrier-free version of ab52962. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247344 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)
    Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)
    All lanes : Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962) at 1/1000 dilution

    Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
    Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) treated with 200nM Okadaic Acid (OA) and 1uM Calyculin A (CA) for 60 min whole cell lysates
    Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) treated with 200nM Okadaic Acid (OA) and 1uM Calyculin A (CA) for 60 min whole cell lysates. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 201 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?


    Exposure time: 90 seconds


    This data was developed using ab52962, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This image is derived from purified batch.

  • Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)
    Western blot - Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)
    All lanes : Anti-Tuberin (phospho S939) antibody [EP1062Y] (ab52962) at 1/10000 dilution

    Lane 1 : 3T3 cell lysate untreated
    Lane 2 : 3T3 cell lysate treated with insulin

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 201 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    This data was developed using ab52962, the same antibody clone in a different buffer formulation.

     

  • Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)
    Anti-Tuberin (phospho S939) antibody [EP1062Y] - BSA and Azide free (ab247344)

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