Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)
![Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)](https://www.abcam.com/ps/products/250/ab250289/Images/ab250289-410270-anti-ndufab1-antibody-epr6725-bsa-and-azide-free-western-blot.jpg "Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6725] to NDUFAB1 - BSA and Azide free
- Suitable for: IP, IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free
See all NDUFAB1 primary antibodies -
Description
Rabbit monoclonal [EPR6725] to NDUFAB1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250289 is the carrier-free version of ab181021. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250289 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6725 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)All lanes : Anti-NDUFAB1 antibody [EPR6725] (ab181021) at 1/10000 dilution
Lane 1 : Molt-4 cell lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDaThis data was developed using ab181021, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)](https://www.abcam.com/ps/products/250/ab250289/Images/ab250289-4-anti-ndufab1-antibody-epr6725-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)This data was developed using ab181021, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human heart tissue sections labeling NDUFAB1 using ab181021 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)](https://www.abcam.com/ps/products/250/ab250289/Images/ab250289-3-anti-ndufab1-antibody-epr6725-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)This data was developed using ab181021, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue sections labeling NDUFAB1 using ab181021 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Flow Cytometry - Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)](https://www.abcam.com/ps/products/250/ab250289/Images/ab250289-2-anti-ndufab1-antibody-epr6725-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)This data was developed using ab181021, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of HepG2 cells labeling NDUFAB1 using ab181021 at a 1/160 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde. -
![Immunoprecipitation - Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)](https://www.abcam.com/ps/products/250/ab250289/Images/ab250289-1-anti-ndufab1-antibody-epr6725-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)This data was developed using ab181021, the same antibody clone in a different buffer formulation.Lysate from HepG2 cells was immunoprecipitated with ab181021 at a 1/60 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST. -
![Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)](https://www.abcam.com/ps/products/250/ab250289/Images/ab250289-6-benefits-of-recombinant-antibodies.png)
Anti-NDUFAB1 antibody [EPR6725] - BSA and Azide free (ab250289)