Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Arc antibody [EPR18950] (ab183183)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18950] to Arc
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Arc antibody [EPR18950]
    See all Arc primary antibodies

  • Description

    Rabbit monoclonal [EPR18950] to Arc

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human hippocampus lysate; Mouse and rat hippocampus and brain lysates; Neuro-2a and SH-SY5Y cell lysates. IHC-P: Mouse hippocampus and cerebral cortex tissues. IP: Mouse brain lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Arc antibody [EPR18950] (ab183183)
    Western blot - Anti-Arc antibody [EPR18950] (ab183183)
    Lanes 1-4 : Anti-Arc antibody [EPR18950] (ab183183) at 1/1000 dilution
    Lane 5 : Anti-Arc antibody [EPR18950] (ab183183) at 1/5000 dilution

    Lane 1 : Mouse hippocampus lysate at 20 µg
    Lane 2 : Rat hippocampus lysate at 20 µg
    Lane 3 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg
    Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg
    Lane 5 : Human hippocampus lysate at 10 µg

    Secondary
    Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Arc antibody [EPR18950] (ab183183)
    Western blot - Anti-Arc antibody [EPR18950] (ab183183)
    All lanes : Anti-Arc antibody [EPR18950] (ab183183) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat liver lysate
    Lane 8 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Arc with ab183183 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Sparse nuclear staining (arrows) in the dentate gyrus of mouse hippocampus (PMID: 19750198, PMID: 19628007). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Arc antibody [EPR18950] (ab183183)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling Arc with ab183183 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on neurons of mouse cerebral cortex. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Arc antibody [EPR18950] (ab183183)
    Immunoprecipitation - Anti-Arc antibody [EPR18950] (ab183183)

    Arc was immunoprecipitated from 0.35 mg of mouse brain lysate with ab183183 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab183183 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse brain lysate 10 µg (Input). 

    Lane 2: ab183183 IP in mouse brain lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183183 in mouse brain lysate.

    Exposure time: 1 second

    Blocking/dilution buffer: 5% NFDM/TBST.

  • Anti-Arc antibody [EPR18950] (ab183183)
    Anti-Arc antibody [EPR18950] (ab183183)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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