ab85022 staining TRPV3 in mouse back skin tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.

Overlay histogram showing SH-SY5Y cells stained with ab85022 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85022, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

All lanes : Anti-TRPV3 antibody [N15/4] (ab85022) at 1 µg/ml

Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from mixture of human cell lines A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G at 10 µg

Predicted band size: 90 kDa

ab85022 staining TRPV3 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.