Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRMT5 antibody [EPR24139-11] - BSA and Azide free (ab280083)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24139-11] to TRMT5 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TRMT5 antibody [EPR24139-11] - BSA and Azide free
    See all TRMT5 primary antibodies

  • Description

    Rabbit monoclonal [EPR24139-11] to TRMT5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat; A431; MCF7; U-2 OS; NIH/3T3 whole cell lysates. ICC: A431; NIH/3T3 cells. Flow cyt: A431; NIH/3T3 cells.

  • General notes

    ab280083 is the carrier-free version of ab259986. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280083 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TRMT5 antibody [EPR24139-11] - BSA and Azide free (ab280083)
    Western blot - Anti-TRMT5 antibody [EPR24139-11] - BSA and Azide free (ab280083)
    All lanes : Anti-TRMT5 antibody [EPR24139-11] (ab259986) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 58 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab259986, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Lane 5 of the blot was developed using a higher sensitivity ECL substrate.

    Exposure time: Lane 1-4: 3 minutes

    Lane 5: 103 seconds

     

  • Immunocytochemistry - Anti-TRMT5 antibody [EPR24139-11] - BSA and Azide free (ab280083)
    Immunocytochemistry - Anti-TRMT5 antibody [EPR24139-11] - BSA and Azide free (ab280083)

    This data was developed using ab259986, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling TRMT5 with ab259986 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weak cytoplasmic staining in A431 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-TRMT5 antibody [EPR24139-11] - BSA and Azide free (ab280083)
    Flow Cytometry - Anti-TRMT5 antibody [EPR24139-11] - BSA and Azide free (ab280083)

    This data was developed using ab259986, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (Human epidermoid carcinoma epithelial cell) cells labelling TRMT5 with ab259986 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
  • Immunocytochemistry - Anti-TRMT5 antibody [EPR24139-11] - BSA and Azide free (ab280083)
    Immunocytochemistry - Anti-TRMT5 antibody [EPR24139-11] - BSA and Azide free (ab280083)

    This data was developed using ab259986, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling TRMT5 with ab259986 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weak cytoplasmic staining in NIH/3T3 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-TRMT5 antibody [EPR24139-11] - BSA and Azide free (ab280083)
    Flow Cytometry - Anti-TRMT5 antibody [EPR24139-11] - BSA and Azide free (ab280083)

    This data was developed using ab259986, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling TRMT5 with ab259986 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

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