Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11832-11] to Calmegin - BSA and Azide free
- Suitable for: ICC, WB, IHC-P, Flow Cyt, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free
See all Calmegin primary antibodies -
Description
Rabbit monoclonal [EPR11832-11] to Calmegin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-P, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee, Common marmoset -
Immunogen
Recombinant fragment within Human Calmegin. The exact sequence is proprietary.
Database link: O14967 -
General notes
Ab249695 is the carrier-free version of ab172477. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249695 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11832-11 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Calmegin antibody [EPR11832-11] (ab172477) at 1/10000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : Rat testis tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDaThis data was developed using ab172477, the same antibody clone in a different buffer formulation.
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This data was developed using ab172477, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Calmegin with ab172477 at 1/250 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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