Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Signal Transduction Protein Trafficking Chaperones Other Chaperones

Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)

Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11832-11] to Calmegin - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Product image
Anti-AIPL1 antibody (ab79039)
Product image
Recombinant Human TCP1 theta protein (ab161115)
Product image
Anti-TCP1 theta antibody (ab176691)
Product image
Anti-TCP1 theta antibody (ab96321)

Overview

  • Product name

    Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free
    See all Calmegin primary antibodies
  • Description

    Rabbit monoclonal [EPR11832-11] to Calmegin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IHC-P, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Common marmoset
  • Immunogen

    Recombinant fragment within Human Calmegin. The exact sequence is proprietary.
    Database link: O14967

  • General notes

    Ab249695 is the carrier-free version of ab172477. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249695 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11832-11
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Developmental Biology
    • Reproduction
    • Germ cell markers

Images

  • Western blot - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)
    Western blot - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)
    All lanes : Anti-Calmegin antibody [EPR11832-11] (ab172477) at 1/10000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Mouse testis tissue lysate
    Lane 4 : Rat testis tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 70 kDa



    This data was developed using ab172477, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)
    This data was developed using ab172477, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Calmegin with ab172477 at 1/250 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)
    Immunocytochemistry - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)
    This data was developed using ab172477, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of Jurkat cells labeling Calmegin with ab172477 at 1/100 dilution.
  • Flow Cytometry - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)
    Flow Cytometry - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)
    This data was developed using ab172477, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells labeling Calmegin with ab172477 at 1/10 dilution (red) compared with a rabbit IgG (negative) (green).
  • Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)
    Immunoprecipitation - Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)
    This data was developed using ab172477, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from Human testis lysate labeling Calmegin with ab172477 at 1/10 dilution.
  • Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)
    Anti-Calmegin antibody [EPR11832-11] - BSA and Azide free (ab249695)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Dopamine Receptor D1 antibody [EPR24102-105] (ab279713)

  •  
  • Product image

    Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] - BSA and Azide free (ab219592)

  •  
  • Product image

    Anti-GRK2 antibody - C-terminal (ab137666)

  •  
  • Product image

    Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)

  •  
  • Product image

    Anti-PHACTR4 antibody [EP12543] - BSA and Azide free (ab269879)

  •  
  • Product image

    Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.