Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CTU2/NCS2 antibody [EPR11194] (ab177160)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11194] to CTU2/NCS2
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-CTU2/NCS2 antibody [EPR11194]
See all CTU2/NCS2 primary antibodies
Description

Rabbit monoclonal [EPR11194] to CTU2/NCS2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CTU2/NCS2 aa 300-400 (Cysteine residue). The exact sequence is proprietary.
Database link: Q2VPK5
Positive control
- HT29, K562, HeLa and Human testis lysates. Human colonic carcinoma and kidney tissues. Permeabilized HT29 cells.
General notes
This product was previously labelled as CTU2

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11194
Isotype

IgG
Research areas

Western blot - Anti-CTU2/NCS2 antibody [EPR11194] (ab177160)

All lanes : Anti-CTU2/NCS2 antibody [EPR11194] (ab177160) at 1/1000 dilution

Lane 1 : HT29 lysate
Lane 2 : K562 lysate
Lane 3 : HeLa lysate
Lane 4 : Human testis lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTU2/NCS2 antibody [EPR11194] (ab177160)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CTU2/NCS2 with ab177160 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-CTU2/NCS2 antibody [EPR11194] (ab177160)

Flow cytometric analysis of permeabilized HT29 cells labeling CTU2/NCS2 with ab177160 at 1/10 dilution (red), or a rabbit IgG (negative) (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTU2/NCS2 antibody [EPR11194] (ab177160)

Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling CTU2/NCS2 with ab177160 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-CTU2/NCS2 antibody [EPR11194] (ab177160)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com