Anti-TRIM29 (acetyl K116) antibody (ab106001)
Key features and details
- Rabbit polyclonal to TRIM29 (acetyl K116)
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
-
Product name
Anti-TRIM29 (acetyl K116) antibody
See all TRIM29 primary antibodies -
Description
Rabbit polyclonal to TRIM29 (acetyl K116) -
Host species
Rabbit -
Specificity
Reacts with over-expressed, acetylated lysine TRIM29. Cross-reactivity with TRIM29 from other sources has not been determined. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRecombinant fragment
-
Immunogen
Synthetic peptide corresponding to an internal portion of Human TRIM29 around lysine 116.
-
Positive control
- 293T cells transfected with acetylated (K116) TRIM29
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-TRIM29 (acetyl K116) antibody (ab106001)All lanes : Anti-TRIM29 (acetyl K116) antibody (ab106001) at 1/500 dilution
Lane 1 : Empty Vector
Lane 2 : Empty Vector plus HDAC inhibitor (TSA)
Lane 3 : TRIM29 WT
Lane 4 : TRIM29 WT plus HDAC inhibitor (TSA)
Lane 5 : TRIM29 K115R
Lane 6 : TRIM29 K115R plus HDAC inhibitor (TSA)
Predicted band size: 66 kDa
Western blot shows detection of a 66 kDa band corresponding to over-expressed, acetylated lysine (K116) TRIM29 (arrowhead) in transfected 293T cells. No staining is noted for cells transfected with empty vector only. No staining is noted for cells transfected with an TRIM29 K116R mutant (K to R transversion lacks site for acetylation). In each instance, samples were prepared with and without TSA (1.3 µM, 6 hr) which inhibits deacetylation.
