Anti-TRIM29 antibody [EPR3494] (ab108627)
![Anti-TRIM29 antibody [EPR3494] (ab108627)](https://www.abcam.com/ps/products/108/ab108627/Images/ab108627-389685-anti-trim29-antibody-epr3494-western-blot-human-lysate.jpg "Anti-TRIM29 antibody [EPR3494] (ab108627)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3494] to TRIM29
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-TRIM29 antibody [EPR3494]
See all TRIM29 primary antibodies -
Description
Rabbit monoclonal [EPR3494] to TRIM29 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, A431, Daudi, 293T, LnCaP, and JAR cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3494 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRIM29 antibody [EPR3494] (ab108627)All lanes : Anti-TRIM29 antibody [EPR3494] (ab108627) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TRIM29 knockout HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 66 kDaLanes 1-4: Merged signal (red and green). Green - ab108627 observed at 74 kDa. Red - loading control ab8245 observed at 37 kDa.
ab108627 Anti-TRIM29 antibody [EPR3494] was shown to specifically react with TRIM29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265878 (knockout cell lysate ab257770) was used. Wild-type and TRIM29 knockout samples were subjected to SDS-PAGE. ab108627 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-TRIM29 antibody [EPR3494] (ab108627)](https://www.abcam.com/ps/products/108/ab108627/Images/ab108627-121378-anti-trim29-antibody-epr3494-western-blot.jpg)
Western blot - Anti-TRIM29 antibody [EPR3494] (ab108627)All lanes : Anti-TRIM29 antibody [EPR3494] (ab108627) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : LnCaP cell lysate
Lane 3 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 66 kDa
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![Anti-TRIM29 antibody [EPR3494] (ab108627)](https://www.abcam.com/ps/products/108/ab108627/Images/ab108627-3-benefits-of-recombinant-antibodies.png)
Anti-TRIM29 antibody [EPR3494] (ab108627)
