Anti-TRIM21/SS-A antibody (ab4369)
Key features and details
- Goat polyclonal to TRIM21/SS-A
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM21/SS-A antibody
See all TRIM21/SS-A primary antibodies -
Description
Goat polyclonal to TRIM21/SS-A -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Synthetic peptide corresponding to TRIM21/SS-A aa 463-475 (N terminal).
Sequence:CPLNIGSQGSTDY
(Peptide available asab23028) -
Positive control
- ICC/IF: U2OS cells. Flow Cyt: HeLa cells.
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General notes
Previously labelled as TRIM21
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TRIM21/SS-A antibody (ab4369)
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling TRIM21/SS-A with ab4369 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
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Flow Cytometry - Anti-TRIM21/SS-A antibody (ab4369)Flow cytometry analysis of HeLa cells labelling TRIM21/SS-A with ab4369 (blue line) at 10 µg/ml. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. Incubated with the primary antibody for 1 hour followed by incubation with Alexa Fluor® 488-conjugated secondary antibody at 1 µg/ml.
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488-conjugated secondary antibody.
