Anti-mouse IgG for IP (HRP) (ab131368)
Key features and details
- Anti-mouse IgG for IP (HRP)
- Conjugation: HRP
- Host species: Rat
- Isotype: IgG
- Suitable for: WB
Overview
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Product name
Anti-mouse IgG for IP (HRP)
See all IgG secondary antibodies -
Host species
Rat -
Target species
Mouse -
Tested applications
Suitable for: WBmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituents: 0.16% Sodium phosphate, 0.88% Sodium chloride, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Isotype
IgG -
General notes
Number of blots:
At least 20 blots, based on a 1 µl/ml dilution and 5 ml diluted antibody per blot.
Important protocol notes:
1. The anti-mouse IgG for IP secondary antibody (HRP) detects mouse IgG antibodies (subtypes: IgG1, IgG2a, IgG2b, IgG3).
2. The anti-mouse IgG for IP secondary antibody (HRP) preferentially detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-denatured forms.
3. IP sample should be completely reduced/denatured before loaded onto a western blot.
4. Milk should be used as the blocking protein for the immunoblot.
Western blot and IP resources:
a) Western blot a beginner's guide
b) IP protocol
c) IP troubleshooting tips -
Research areas
Images
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Western blot - Anti-mouse IgG for IP (HRP) (ab131368)
IP sample preparation: Caspase 7 was immunoprecipitated from 0.5 ml of 1x107 Jurkat (Human T cell leukemia cell line from peripheral blood) cells/ml with 5 µg mouse anti-human Caspase 7.
WB conditions: Precipitate from 1x106 cells was subjected to electrophoresis, transferred to an PVDF membrane, and immunoblotted with an anti-Caspase 7 antibody.
Detection:
Lane 1: Detection with anti-mouse IgG for IP secondary antibody (HRP) (ab131368)
Lane 2: Detection with an HRP-conjugated anti-mouse IgG H&L secondary antibody
Lane 3: Lane 1 was re-immunoblotted using an HRP-conjugated anti-mouse IgG H&L secondary antibody. The heavy and light-chains can now be seen, confirming that although the immunoprecipitating heavy and light-chains are present, ab131368 detects only native antibody and not denatured heavy and light-chains.Please note the detection of the heavy and light-chains of the immunoprecipitating antibody in Lane 2 but not in Lane 1.