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Anti-TREX1 antibody [EPR14985] (ab185228)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14985] to TREX1
  • Suitable for: ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-TREX1 antibody [EPR14985]
    See all TREX1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14985] to TREX1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, Raji, Daudi and HeLa whole cell lysate (ab150035). IHC-P: Human colon and adenocarcinoma of colon tissue. ICC: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TREX1 antibody [EPR14985] (ab185228)
    Western blot - Anti-TREX1 antibody [EPR14985] (ab185228)
    All lanes : Anti-TREX1 antibody [EPR14985] (ab185228) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : TREX1 knockout A549 cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 39 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab185228 observed at 34 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab185228 Anti-TREX1 antibody [EPR14985] was shown to specifically react with TREX1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266927 (knockout cell lysate ab257764) was used. Wild-type and TREX1 knockout samples were subjected to SDS-PAGE. ab185228 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-TREX1 antibody [EPR14985] (ab185228)
    Immunocytochemistry/ Immunofluorescence - Anti-TREX1 antibody [EPR14985] (ab185228)

    Confocal image showing cytoplasmic and weak nuclear staining in HeLa (human cervix adenocarcinoma epithelial cell) cells.  Cells were fixed in 4% paraformaldehyde and permeabilised with 0.1% TritonX-100.  The primary anti-TREX1 antibody, ab185228, was used at a 1:200 dilution (10 μg/ml).  An AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) was used at a 1:1000 dilution (2 μg/ml) (green).  An anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as a counterstain at a 1:200 dilution (2.5 μg/ml) (red).  DAPI (blue) was used as a nuclear counterstain.  A secondary antibody only control was also performed.

  • Western blot - Anti-TREX1 antibody [EPR14985] (ab185228)
    Western blot - Anti-TREX1 antibody [EPR14985] (ab185228)
    All lanes : Anti-TREX1 antibody [EPR14985] (ab185228) at 1/10000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : Daudi cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREX1 antibody [EPR14985] (ab185228)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREX1 antibody [EPR14985] (ab185228)

    Immunohistochemical analysis of paraffin embedded human colon adenocarcinoma tissue sections labeling TREX1 using ab185228 at a 1/100 dilution. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREX1 antibody [EPR14985] (ab185228)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREX1 antibody [EPR14985] (ab185228)

    Immunohistochemical analysis of paraffin embedded Human colon tissue sections labeling TREX1 using ab185228 at a 1/100 dilution. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-TREX1 antibody [EPR14985] (ab185228)
    Anti-TREX1 antibody [EPR14985] (ab185228)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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