Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TREX1 antibody [EPR14985] - BSA and Azide free (ab236140)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14985] to TREX1 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-TREX1 antibody [EPR14985] - BSA and Azide free
    See all TREX1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14985] to TREX1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon adenocarcinoma tissue. WB: A549, Raji and Daudi cell lysates. ICC: HeLa cells.

  • General notes

    Ab236140 is the carrier-free version of ab185228. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236140 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-TREX1 antibody [EPR14985] - BSA and Azide free (ab236140)
    Western blot - Anti-TREX1 antibody [EPR14985] - BSA and Azide free (ab236140)
    All lanes : Anti-TREX1 antibody [EPR14985] (ab185228) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : TREX1 knockout A549 cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 39 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab185228, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab185228 observed at 34 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab185228 Anti-TREX1 antibody [EPR14985] was shown to specifically react with TREX1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266927 (knockout cell lysate ab257764) was used. Wild-type and TREX1 knockout samples were subjected to SDS-PAGE. ab185228 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREX1 antibody [EPR14985] - BSA and Azide free (ab236140)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREX1 antibody [EPR14985] - BSA and Azide free (ab236140)

    Immunohistochemical analysis of paraffin embedded Human colon tissue sections labeling TREX1 using ab185228 at a 1/100 dilution. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185228).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TREX1 antibody [EPR14985] - BSA and Azide free (ab236140)
    Immunocytochemistry/ Immunofluorescence - Anti-TREX1 antibody [EPR14985] - BSA and Azide free (ab236140)

    Immunofluorescent analysis of -20°C Acetone fixed HeLa cells labeling TREX1 using ab185228 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185228).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREX1 antibody [EPR14985] - BSA and Azide free (ab236140)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREX1 antibody [EPR14985] - BSA and Azide free (ab236140)

    Immunohistochemical analysis of paraffin embedded human colon adenocarcinoma tissue sections labeling TREX1 using ab185228 at a 1/100 dilution. Hematoxylin counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185228).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-TREX1 antibody [EPR14985] - BSA and Azide free (ab236140)
    Anti-TREX1 antibody [EPR14985] - BSA and Azide free (ab236140)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-TREX1 antibody [EPR14985] - BSA and Azide free (ab236140)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.