Peroxidase Activity Assay Kit (ab155895)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine
Overview
-
Product name
Peroxidase Activity Assay Kit
See all HRP kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids -
Assay type
Enzyme activity -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam’s Peroxidase Assay Kit (ab155895) provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the redfluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (λmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.
-
Notes
Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ → ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests HRP Positive Control Green 1 vial Hydrogen Peroxide Substrate Yellow 1 x 0.1ml OxiRed Probe Red 1 x 0.2ml Peroxidase Assay Buffer WM 1 x 25ml -
Research areas
-
Relevance
Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. -
Cellular localization
Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles. -
Alternative names
- Horseradish Peroxidase
- HPRC1
- HRP
see all
Images
-
H2O2 standard curve - fluorometricAn example of a Fluorimetric Standard Curve
-
H2O2 Standard Curve - ColorimetricAn example of a Colorimetric Standard Curve