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Signal Transduction Metabolism Drug metabolism

Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (ab249390)

Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (ab249390)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10821(B)] to TPMT - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB
  • Knockout validated
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free
    See all TPMT primary antibodies
  • Description

    Rabbit monoclonal [EPR10821(B)] to TPMT - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249390 is the carrier-free version of ab166909.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10821(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism

Images

  • Western blot - Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (ab249390)
    Western blot - Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (ab249390)
    All lanes : Anti-TPMT antibody [EPR10821(B)] (ab166909) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : TPMT knockout HAP1 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : K562 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 28 kDa



    This data was developed using ab166909, the same antibody clone in a different buffer formulation.

    Lanes 1 - 4: Merged signal (red and green). Green - ab166909 observed at 28 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab166909 was shown to recognize TPMT in wild-type HAP1 cells as signal was lost at the expected MW in TPMT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TPMT knockout samples were subjected to SDS-PAGE. ab166909 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (ab249390)
    Western blot - Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (ab249390)
    All lanes : Anti-TPMT antibody [EPR10821(B)] (ab166909) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : TF-1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 28 kDa



    This data was developed using ab166909, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (ab249390)
    Flow Cytometry - Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (ab249390)
    This data was developed using ab166909, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized K562 cells using ab166909 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).
  • Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (ab249390)
    Anti-TPMT antibody [EPR10821(B)] - BSA and Azide free (ab249390)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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