Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UGT3A1 antibody [EPR13723] (ab181245)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13723] to UGT3A1
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-UGT3A1 antibody [EPR13723]
    See all UGT3A1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13723] to UGT3A1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human testis, fetal liver and fetal kidney cell lysates; HepG2 cell lysate; Human liver and kidney tissues; HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-UGT3A1 antibody [EPR13723] (ab181245)
    Western blot - Anti-UGT3A1 antibody [EPR13723] (ab181245)
    All lanes : Anti-UGT3A1 antibody [EPR13723] (ab181245) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-UGT3A1 antibody [EPR13723] (ab181245)
    Immunocytochemistry/ Immunofluorescence - Anti-UGT3A1 antibody [EPR13723] (ab181245)

    Immunofluorescence analysis of, paraformaldehyde-fixed, HepG2 cells labeling UGT3A1 with ab181245 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.

  • Western blot - Anti-UGT3A1 antibody [EPR13723] (ab181245)
    Western blot - Anti-UGT3A1 antibody [EPR13723] (ab181245)
    Anti-UGT3A1 antibody [EPR13723] (ab181245) at 1/1000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] (ab181245)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] (ab181245)

    Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling UGT3A1 with ab181245 at a 1/100 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] (ab181245)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT3A1 antibody [EPR13723] (ab181245)

    Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling UGT3A1 with ab181245 at a 1/100 dilution. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-UGT3A1 antibody [EPR13723] (ab181245)
    Immunoprecipitation - Anti-UGT3A1 antibody [EPR13723] (ab181245)

    Western blot analysis on immunoprecipitation from 1) Human fetal liver lysate or 2) negative control, labeling UGT3A1 using ab181245 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

  • Anti-UGT3A1 antibody [EPR13723] (ab181245)
    Anti-UGT3A1 antibody [EPR13723] (ab181245)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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