Anti-Topoisomerase III alpha antibody (ab108493)
Key features and details
- Rabbit polyclonal to Topoisomerase III alpha
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Topoisomerase III alpha antibody -
Description
Rabbit polyclonal to Topoisomerase III alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- This antibody gave a positive signal in both HeLa and HEK293 whole cell lysates. This antibody gave a positive result in IF in the following Methanol fixed cell line: HeLa.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications ChimpanzeeGorillaApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 112 kDa).ICC/IF Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 112 kDa).ICC/IF
Use a concentration of 1 µg/ml.Target
-
Function
Reduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. -
Tissue specificity
High expression is found in testis, heart, skeletal muscle and pancreas. -
Sequence similarities
Belongs to the prokaryotic type I/III topoisomerase family.
Contains 1 Toprim domain. - Information by UniProt
-
Database links
- Entrez Gene: 7156 Human
- Omim: 601243 Human
- SwissProt: Q13472 Human
- Unigene: 592115 Human
-
Alternative names
- DNA topoisomerase 3 alpha antibody
- DNA topoisomerase 3-alpha antibody
- DNA topoisomerase III alpha antibody
see all
Images
-
Western blot - Anti-Topoisomerase III alpha antibody (ab108493)All lanes : Anti-Topoisomerase III alpha antibody (ab108493) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase III alpha antibody (ab108493)ab108493 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108493 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (0)
ab108493 has not yet been referenced specifically in any publications.
Images
-
Western blot - Anti-Topoisomerase III alpha antibody (ab108493)All lanes : Anti-Topoisomerase III alpha antibody (ab108493) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
-
Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase III alpha antibody (ab108493)
ab108493 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108493 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
