Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6932(2)] to TOMM40
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-TOMM40 antibody [EPR6932(2)]
    See all TOMM40 primary antibodies

  • Description

    Rabbit monoclonal [EPR6932(2)] to TOMM40

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa and 293T cell lysate. Human liver tissue. HeLa and 293T cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
    Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
    All lanes : Anti-TOMM40 antibody [EPR6932(2)] (ab185543) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
    Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

    Western blot analysis of TOMM40 in immunoprecipitation pellets from HeLa lysate. ab185543 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.

  • Flow Cytometry - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
    Flow Cytometry - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

    Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling TOMM40 with ab185543 at 1/300 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
    Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

    Immunofluorescent analysis of 4% paraformaldehyde fixed 293T cells labeling TOMM40 with ab185543 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOMM40 with ab185543 at 1/500 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-TOMM40 antibody [EPR6932(2)] (ab185543)
    Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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