Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6932(2)] to TOMM40
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
Reacts with: Human

Product name

Anti-TOMM40 antibody [EPR6932(2)]
See all TOMM40 primary antibodies
Description

Rabbit monoclonal [EPR6932(2)] to TOMM40
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa and 293T cell lysate. Human liver tissue. HeLa and 293T cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

EPR6932(2)
Isotype

IgG
Research areas

Western blot - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

All lanes : Anti-TOMM40 antibody [EPR6932(2)] (ab185543) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa
Observed band size: 38 kDa
Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

Western blot analysis of TOMM40 in immunoprecipitation pellets from HeLa lysate. ab185543 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.
Flow Cytometry - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling TOMM40 with ab185543 at 1/300 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

Immunofluorescent analysis of 4% paraformaldehyde fixed 293T cells labeling TOMM40 with ab185543 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOMM40 with ab185543 at 1/500 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-TOMM40 antibody [EPR6932(2)] (ab185543)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com