Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6932(2)] to TOMM40 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, IP, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free
    See all TOMM40 primary antibodies

  • Description

    Rabbit monoclonal [EPR6932(2)] to TOMM40 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HeLa cell lysate. IHC-P: Human liver tissue. Flow Cyt: HeLa cells. ICC/IF: 293T cells.

  • General notes

    Ab240964 is the carrier-free version of ab185543. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240964 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOMM40 with ab185543 at 1/500 dilution. Counter stain Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185543).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)
    Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)

    Immunofluorescent analysis of 4% paraformaldehyde fixed 293T cells labeling TOMM40 with ab185543 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185543).

  • Flow Cytometry - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)
    Flow Cytometry - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)

    Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling TOMM40 with ab185543 at 1/300 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185543).

  • Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)
    Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)

    Western blot analysis of TOMM40 in immunoprecipitation pellets from HeLa lysate. ab185543 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185543).

  • Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)
    Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)

© 2021 Astana Biomed Group LLP. All rights reserved.