Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)
![Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)](https://www.abcam.com/ps/products/240/ab240964/Images/ab240964-4-ab185543219845ab1855432.jpg "Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6932(2)] to TOMM40 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, IP, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free
See all TOMM40 primary antibodies -
Description
Rabbit monoclonal [EPR6932(2)] to TOMM40 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, IP, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: HeLa cell lysate. IHC-P: Human liver tissue. Flow Cyt: HeLa cells. ICC/IF: 293T cells.
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General notes
Ab240964 is the carrier-free version of ab185543. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240964 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6932(2) -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOMM40 with ab185543 at 1/500 dilution. Counter stain Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185543).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)](https://www.abcam.com/ps/products/240/ab240964/Images/ab240964-3-ab185543219846ab1855433.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)Immunofluorescent analysis of 4% paraformaldehyde fixed 293T cells labeling TOMM40 with ab185543 at 1/100 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185543).
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![Flow Cytometry - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)](https://www.abcam.com/ps/products/240/ab240964/Images/ab240964-2-ab185543219847ab1855434.jpg)
Flow Cytometry - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling TOMM40 with ab185543 at 1/300 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185543).
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![Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)](https://www.abcam.com/ps/products/240/ab240964/Images/ab240964-1-ab185543219848ab1855435.jpg)
Immunoprecipitation - Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)Western blot analysis of TOMM40 in immunoprecipitation pellets from HeLa lysate. ab185543 used at 1/50 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185543).
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![Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)](https://www.abcam.com/ps/products/240/ab240964/Images/ab240964-5-benefits-of-recombinant-antibodies.png)
Anti-TOMM40 antibody [EPR6932(2)] - BSA and Azide free (ab240964)
