Anti-TNFRSF14/HVEM antibody [EPR22122] - BSA and Azide free (ab251647)
![Anti-TNFRSF14/HVEM antibody [EPR22122] - BSA and Azide free (ab251647)](https://www.abcam.com/ps/products/251/ab251647/Images/ab251647-3-224708FC2.jpg "Anti-TNFRSF14/HVEM antibody [EPR22122] - BSA and Azide free (ab251647)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22122] to TNFRSF14/HVEM - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-TNFRSF14/HVEM antibody [EPR22122] - BSA and Azide free
See all TNFRSF14/HVEM primary antibodies -
Description
Rabbit monoclonal [EPR22122] to TNFRSF14/HVEM - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HEK-293T cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct. Flow cyt: Human PBMCs and HEK-293T cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct.
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General notes
ab251647 is the carrier-free version of ab224708 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251647 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as TNFRSF14
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22122 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-TNFRSF14/HVEM antibody [EPR22122] - BSA and Azide free (ab251647)
Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct labeling TNFRSF14/HVEM with ab224708 at 1/400 dilution (red) compared with a rabbit monoclonal IgG Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224708).
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![Flow Cytometry - Anti-TNFRSF14/HVEM antibody [EPR22122] - BSA and Azide free (ab251647)](https://www.abcam.com/ps/products/251/ab251647/Images/ab251647-2-224708FC1.jpg)
Flow Cytometry - Anti-TNFRSF14/HVEM antibody [EPR22122] - BSA and Azide free (ab251647)Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling TNFRSF14/HVEM with ab224708 at 1/400 dilution (red) compared with a rabbit monoclonal IgG Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224708).
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![Immunocytochemistry/ Immunofluorescence - Anti-TNFRSF14/HVEM antibody [EPR22122] - BSA and Azide free (ab251647)](https://www.abcam.com/ps/products/251/ab251647/Images/ab251647-1-224708ICC1a.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TNFRSF14/HVEM antibody [EPR22122] - BSA and Azide free (ab251647)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with with a Myc-tagged human TNFRSF14/HVEM expression construct labeling TNFRSF14/HVEM with ab224708 at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with a Myc-tagged human TNFRSF14/HVEM expression construct. The nuclear counter stain is DAPI (blue). Cells are counter stained for Myc using Anti-Myc tag antibody [9E10] (Alexa Fluor® 594) (ab223894) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224708).
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![Anti-TNFRSF14/HVEM antibody [EPR22122] - BSA and Azide free (ab251647)](https://www.abcam.com/ps/products/251/ab251647/Images/ab251647-4-benefits-of-recombinant-antibodies.png)
Anti-TNFRSF14/HVEM antibody [EPR22122] - BSA and Azide free (ab251647)
