Anti-TMEM9B antibody [EPR14339] (ab189253)
![Anti-TMEM9B antibody [EPR14339] (ab189253)](https://www.abcam.com/ps/products/189/ab189253/Images/ab189253-224612-anti-tmem9b-antibody-epr14339-western-blot.jpg "Anti-TMEM9B antibody [EPR14339] (ab189253)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14339] to TMEM9B
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TMEM9B antibody [EPR14339]
See all TMEM9B primary antibodies -
Description
Rabbit monoclonal [EPR14339] to TMEM9B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB MouseHuman
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Immunogen
This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment within Human TMEM9B aa 100 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9NQ34 -
Positive control
- HeLa, HepG2, BxPC-3, Mouse brain, rat brain lysates; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14339 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TMEM9B antibody [EPR14339] (ab189253)All lanes : Anti-TMEM9B antibody [EPR14339] (ab189253) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-TMEM9B antibody [EPR14339] (ab189253)](https://www.abcam.com/ps/products/189/ab189253/Images/ab189253-224610-anti-tmem9b-antibody-epr14339-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TMEM9B antibody [EPR14339] (ab189253)Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling TMEM9B with ab189253 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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![Western blot - Anti-TMEM9B antibody [EPR14339] (ab189253)](https://www.abcam.com/ps/products/189/ab189253/Images/ab189253-224611-anti-tmem9b-antibody-epr14339-western-blot.jpg)
Western blot - Anti-TMEM9B antibody [EPR14339] (ab189253)All lanes : Anti-TMEM9B antibody [EPR14339] (ab189253) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
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![Anti-TMEM9B antibody [EPR14339] (ab189253)](https://www.abcam.com/ps/products/189/ab189253/Images/ab189253-4-benefits-of-recombinant-antibodies.png)
Anti-TMEM9B antibody [EPR14339] (ab189253)
