Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TMEM9B antibody [EPR14339] (ab189253)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14339] to TMEM9B
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TMEM9B antibody [EPR14339]
    See all TMEM9B primary antibodies

  • Description

    Rabbit monoclonal [EPR14339] to TMEM9B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    Recombinant fragment within Human TMEM9B aa 100 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9NQ34

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa, HepG2, BxPC-3, Mouse brain, rat brain lysates; HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14339

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TMEM9B antibody [EPR14339] (ab189253)
    Western blot - Anti-TMEM9B antibody [EPR14339] (ab189253)
    All lanes : Anti-TMEM9B antibody [EPR14339] (ab189253) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : BxPC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TMEM9B antibody [EPR14339] (ab189253)
    Immunocytochemistry/ Immunofluorescence - Anti-TMEM9B antibody [EPR14339] (ab189253)

    Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling TMEM9B with ab189253 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Western blot - Anti-TMEM9B antibody [EPR14339] (ab189253)
    Western blot - Anti-TMEM9B antibody [EPR14339] (ab189253)
    All lanes : Anti-TMEM9B antibody [EPR14339] (ab189253) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa

  • Anti-TMEM9B antibody [EPR14339] (ab189253)
    Anti-TMEM9B antibody [EPR14339] (ab189253)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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