Anti-TMEM43 antibody [EPR15379] (ab190793)
![Anti-TMEM43 antibody [EPR15379] (ab190793)](https://www.abcam.com/ps/products/190/ab190793/Images/ab190793-226972-ab190793IHC.jpg "Anti-TMEM43 antibody [EPR15379] (ab190793)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15379] to TMEM43
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Overview
-
Product name
Anti-TMEM43 antibody [EPR15379]
See all TMEM43 primary antibodies -
Description
Rabbit monoclonal [EPR15379] to TMEM43 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatHumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Caco-2 cells; Rat liver tissue; Human transitional cell carcinoma of bladder tissue; Human spleen, placenta and ovary cancer lysates; JAR, MDA-MB-435 and Caco-2 cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15379 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15379] (ab190793)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder labeling TMEM43 with ab190793 at 1/250 dilution followed by prediluted HRP polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15379] (ab190793)](https://www.abcam.com/ps/products/190/ab190793/Images/ab190793-226974-ab190793IHCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15379] (ab190793)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling TMEM43 with ab190793 at 1/250 dilution followed by prediluted HRP polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Western blot - Anti-TMEM43 antibody [EPR15379] (ab190793)](https://www.abcam.com/ps/products/190/ab190793/Images/ab190793-226971-ab190793WB.jpg)
Western blot - Anti-TMEM43 antibody [EPR15379] (ab190793)All lanes : Anti-TMEM43 antibody [EPR15379] (ab190793) at 1/1000 dilution
Lane 1 : Human spleen lysate
Lane 2 : Human placenta lysate
Lane 3 : Human ovary cancer lysate
Lane 4 : JAR cell lysate
Lane 5 : MDA-MB-435 cell lysate
Lane 6 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
-
![Anti-TMEM43 antibody [EPR15379] (ab190793)](https://www.abcam.com/ps/products/190/ab190793/Images/ab190793-5-benefits-of-recombinant-antibodies.png)
Anti-TMEM43 antibody [EPR15379] (ab190793)
