Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15378(B)] to TMEM43
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-TMEM43 antibody [EPR15378(B)]
See all TMEM43 primary antibodies -
Description
Rabbit monoclonal [EPR15378(B)] to TMEM43 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human spleen and placenta lysates; MDA-MB-435 and Jurkat cell lysates; Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15378(B) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-TMEM43 antibody [EPR15378(B)] (ab184164) at 1/5000 dilution
Lane 1 : Human spleen lysates
Lane 2 : Human placenta lysates
Lane 3 : MDA-MB-435 cell lysates
Lane 4 : Jurkat cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
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Anti-TMEM43 antibody [EPR15378(B)] (ab184164) at 1/20000 dilution + CaCo-2 cell lysates at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDa
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TMEM43 with ab184164 at 1/500 dilution. The slide is counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TMEM43 with ab184164 at 1/500 dilution. The slide is counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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