Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (ab250454)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12842] to TMEM256 - BSA and Azide free
  • Suitable for: IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TMEM256 antibody [EPR12842] - BSA and Azide free
    See all TMEM256 primary antibodies

  • Description

    Rabbit monoclonal [EPR12842] to TMEM256 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250454 is the carrier-free version of ab181239 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250454 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as C17orf61

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12842

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (ab250454)
    Western blot - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (ab250454)
    All lanes : Anti-TMEM256 antibody [EPR12842] (ab181239) at 1/10000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : Y79 cell lysate
    Lane 3 : LnCaP cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 12 kDa



    This data was developed using ab181239, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (ab250454)
    Flow Cytometry - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (ab250454)
    This data was developed using ab181239, the same antibody clone in a different buffer formulation.Flow cytometric analysis of A549 cells (paraformaldehyde-fixed, 2%) labeling TMEM256 with ab181239 at 1/200 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
  • Immunoprecipitation - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (ab250454)
    Immunoprecipitation - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (ab250454)
    This data was developed using ab181239, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab181239 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
  • Western blot - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (ab250454)
    Western blot - Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (ab250454)
    All lanes : Anti-TMEM256 antibody [EPR12842] (ab181239) at 1/2000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 12 kDa



    This data was developed using ab181239, the same antibody clone in a different buffer formulation.

     

  • Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (ab250454)
    Anti-TMEM256 antibody [EPR12842] - BSA and Azide free (ab250454)

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