Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4510] to S100 Calcium Binding Protein A13/S100A13 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt (Intra), IP, ICC/IF, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free
    See all S100 Calcium Binding Protein A13/S100A13 primary antibodies

  • Description

    Rabbit monoclonal [EPR4510] to S100 Calcium Binding Protein A13/S100A13 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt (Intra), IP, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HUVEC cells Flow Cyt (intra): HUVEC cells IP: Human heart lysate. WB: HEK-293T and HeLa cell lysates.

  • General notes

    ab239962 is the carrier-free version of ab109252.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Images

  • Western blot - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)
    Western blot - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)
    All lanes : Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : S100A13 knockout HEK-293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab109252).

    Lanes 1 - 4: Merged signal (red and green). Green - ab109252 observed at 13 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab109252 was shown to react with S100 Calcium Binding Protein A13/S100A13 in wild-type HEK-293T cells in Western blot with loss of signal observed in S100A13 knockout cell line ab266065 (S100A13 knockout cell lysate ab258183). Wild-type HEK-293T and S100A13 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab109252 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)
    Immunocytochemistry/ Immunofluorescence - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)

    Immunocytochemistry/ Immunofluorescence analysis of HUVEC (human umbilical vein endothelial cell) cells labeling S100 Calcium Binding Protein A13/S100A13 with purified ab109252 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109252).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)

    ab109252, at a 1/250 dilution, staining S100 Calcium Binding Protein A13/S100A13 in Human brain, by Immunohistochemistry, Paraffin-embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109252).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)
    Flow Cytometry - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)
    This data was developed using ab109252, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HUVEC (Human umbilical vein endothelial cell) cells labeling S100 Calcium Binding Protein A13/S100A13 with purified ab109252 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Immunoprecipitation - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)
    Immunoprecipitation - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)

    This data was developed using ab109252, the same antibody clone in a different buffer formulation.
    Purified ab109252 at 1/50 dilution (2µg) immunoprecipitating S100 Calcium Binding Protein A13/S100A13 in Human heart lysate.
    Lane 1 (input): Human heart lysate 10µg
    Lane 2 (+): ab109252 + Human heart lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109252 in Human heart lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 11 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)

    ab109252, at a 1/250 dilution, staining S100 Calcium Binding Protein A13/S100A13 in Human muscle, by Immunohistochemistry, Paraffin-embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109252).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)
    Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] - BSA and Azide free (ab239962)

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