Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4510] to S100 Calcium Binding Protein A13/S100A13
  • Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510]
    See all S100 Calcium Binding Protein A13/S100A13 primary antibodies

  • Description

    Rabbit monoclonal [EPR4510] to S100 Calcium Binding Protein A13/S100A13

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • EB: HeLa, HEK293T, HUVEC and ECV304 cell lysates; Human thyroid and testis tissue lysates. IHC-P: Human brain and muscle tissues. ICC//IF: HUVEC cells Flow Cyt: HUVEC cells IP: Human heart lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product was previously labelled as S100 Calcium Binding Protein A13

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)
    Western blot - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)
    All lanes : Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : S100A13 knockout HEK-293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab109252 observed at 13 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab109252 was shown to react with S100 Calcium Binding Protein A13/S100A13 in wild-type HEK-293T cells in Western blot with loss of signal observed in S100A13 knockout cell line ab266065 (S100A13 knockout cell lysate ab258183). Wild-type HEK-293T and S100A13 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab109252 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)
    Immunocytochemistry/ Immunofluorescence - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

    Immunocytochemistry/ Immunofluorescence analysis of HUVEC (human umbilical vein endothelial cell) cells labeling S100 Calcium Binding Protein A13/S100A13 with purified ab109252 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

    ab109252, at a 1/250 dilution, staining S100 Calcium Binding Protein A13/S100A13 in Human brain, by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)
    Flow Cytometry - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)
    Flow Cytometry analysis of HUVEC (Human umbilical vein endothelial cell) cells labeling S100 Calcium Binding Protein A13/S100A13 with purified ab109252 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Western blot - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)
    Western blot - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)
    All lanes : Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252) at 1/1000 dilution

    Lane 1 : Human thyroid tissue lysate
    Lane 2 : Human testis tissue lysate
    Lane 3 : HUVEC lysate
    Lane 4 : ECV304 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 11 kDa

  • Immunoprecipitation - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)
    Immunoprecipitation - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

    Purified ab109252 at 1/50 dilution (2µg) immunoprecipitating S100 Calcium Binding Protein A13/S100A13 in Human heart lysate.
    Lane 1 (input): Human heart lysate 10µg
    Lane 2 (+): ab109252 + Human heart lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109252 in Human heart lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 11 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

    ab109252, at a 1/250 dilution, staining S100 Calcium Binding Protein A13/S100A13 in Human muscle, by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)
    Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

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