Antibodies, Proteins, Kits and Reagents for Life Science

237 878 ₸ Product size

100 µl 237 878 ₸ 10 µl 97 161 ₸

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Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4510] to S100 Calcium Binding Protein A13/S100A13
Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510]
See all S100 Calcium Binding Protein A13/S100A13 primary antibodies
Description

Rabbit monoclonal [EPR4510] to S100 Calcium Binding Protein A13/S100A13
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- EB: HeLa, HEK293T, HUVEC and ECV304 cell lysates; Human thyroid and testis tissue lysates. IHC-P: Human brain and muscle tissues. ICC//IF: HUVEC cells Flow Cyt: HUVEC cells IP: Human heart lysate.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product was previously labelled as S100 Calcium Binding Protein A13

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4510
Isotype

IgG
Research areas

Western blot - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

All lanes : Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : S100A13 knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Daudi cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 11 kDa
Observed band size: 13 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab109252 observed at 13 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab109252 was shown to react with S100 Calcium Binding Protein A13/S100A13 in wild-type HEK-293T cells in Western blot with loss of signal observed in S100A13 knockout cell line ab266065 (S100A13 knockout cell lysate ab258183). Wild-type HEK-293T and S100A13 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab109252 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

Immunocytochemistry/ Immunofluorescence analysis of HUVEC (human umbilical vein endothelial cell) cells labeling S100 Calcium Binding Protein A13/S100A13 with purified ab109252 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

ab109252, at a 1/250 dilution, staining S100 Calcium Binding Protein A13/S100A13 in Human brain, by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

Flow Cytometry analysis of HUVEC (Human umbilical vein endothelial cell) cells labeling S100 Calcium Binding Protein A13/S100A13 with purified ab109252 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

All lanes : Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252) at 1/1000 dilution

Lane 1 : Human thyroid tissue lysate
Lane 2 : Human testis tissue lysate
Lane 3 : HUVEC lysate
Lane 4 : ECV304 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 11 kDa
Immunoprecipitation - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

Purified ab109252 at 1/50 dilution (2µg) immunoprecipitating S100 Calcium Binding Protein A13/S100A13 in Human heart lysate.
Lane 1 (input): Human heart lysate 10µg
Lane 2 (+): ab109252 + Human heart lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109252 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 11 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

ab109252, at a 1/250 dilution, staining S100 Calcium Binding Protein A13/S100A13 in Human muscle, by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-S100 Calcium Binding Protein A13/S100A13 antibody [EPR4510] (ab109252)

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