Human FGF19 ELISA Kit (ab230943)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.4 pg/ml
- Range: 3.91 pg/ml - 250 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human FGF19 ELISA Kit
See all FGF19 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 5 3.8% Inter-assay Sample n Mean SD CV% Supernatant 3 6.6% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.4 pg/ml -
Range
3.91 pg/ml - 250 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 112 106% - 117% Cell culture media 108 107% - 110% Hep Plasma 108 101% - 116% EDTA Plasma 94% - 99% Cit plasma 100 97% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human FGF19 ELISA Kit (ab230943) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF19 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FGF19 with 1.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human FGF19 Capture Antibody 1 x 600µl 10X Human FGF19 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human FGF19 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB. -
Tissue specificity
Expressed in fetal brain, cartilage, retina, and adult gall bladder. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- FGF 19
- FGF-19
- FGF15
see all -
Database links
- Entrez Gene: 9965 Human
- NCBI: NM_005117 Human
- Omim: 603891 Human
- SwissProt: O95750 Human
- Unigene: 249200 Human
Images
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Other - Human FGF19 ELISA Kit (ab230943)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human FGF19 Competitor Std Curve ComparisonStandard curve comparison between human FGF19 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human FGF19 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native FGF19 in human serum, and plasma samplesThe concentrations of FGF19 were measured in duplicates, interpolated from the FGF19 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5% and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF19 concentration was determined to be 153 pg/mL in serum , 91 pg/mL in plasma (EDTA), 187 pg/mL in plasma (heparin), and 182 pg/mL in plasma (citrate).
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Interpolated concentrations of spike FGF19 in human serum, and plasma samplesThe concentrations of FGF19 were measured in duplicates, interpolated from the FGF19 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 12.5%, plasma (EDTA) 12.5% and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native FGF19 in HT-29 cell culture supernatantThe concentrations of FGF19 were measured in duplicates, interpolated from the FGF19 standard curves and corrected for sample dilution. Undiluted samples are as follows: HT-29 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF19 concentration was determined to be 741 pg/mL in HT-29 cell culture supernatant.
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Serum from ten individual healthy human male donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF19 concentration was determined to be 381 pg/mL with a range of 136 – 944 pg/mL.
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Sandwich ELISA - Human FGF19 ELISA Kit (ab230943)To learn more about the advantages of recombinant antibodies see here.
