Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TLR3 antibody [EPR24454-103] - BSA and Azide free (ab281567)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24454-103] to TLR3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Human

Overview

  • Product name

    Anti-TLR3 antibody [EPR24454-103] - BSA and Azide free
    See all TLR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR24454-103] to TLR3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra)more details
    Unsuitable for: ICC,IHC-P,IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow cyt: Human PBMC treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 6 days.

  • General notes

    ab281567 is the carrier-free version of ab281560. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281567 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24454-103

  • Isotype

    IgG

Images

  • Flow Cytometry - Anti-TLR3 antibody [EPR24454-103] - BSA and Azide free (ab281567)
    Flow Cytometry - Anti-TLR3 antibody [EPR24454-103] - BSA and Azide free (ab281567)

    This data was developed using ab281560, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 6 days cells labelling TLR3 with ab281560 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD11c conjugated to Alexa Fluor® 647 and anti-HLA-DR conjugated to PE, then stained with rabbit IgG or ab281560.Gated on HLA-DR+ population.
  • Flow Cytometry - Anti-TLR3 antibody [EPR24454-103] - BSA and Azide free (ab281567)
    Flow Cytometry - Anti-TLR3 antibody [EPR24454-103] - BSA and Azide free (ab281567)

    This data was developed using ab281560, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Human peripheral blood mononuclear cell (PBMC) treated with 100ng/ml GM-CSF and 50ng/ml IL-4 for 6 days cells labelling TLR3 with ab281560 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
  • Anti-TLR3 antibody [EPR24454-103] - BSA and Azide free (ab281567)
    Anti-TLR3 antibody [EPR24454-103] - BSA and Azide free (ab281567)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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