Anti-TIM50 antibody (ab204486)
Key features and details
- Rabbit polyclonal to TIM50
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TIM50 antibody
See all TIM50 primary antibodies -
Description
Rabbit polyclonal to TIM50 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
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Immunogen
Recombinant fragment corresponding to Human TIM50 aa 150-250.
Database link: Q3ZCQ8 -
Positive control
- IHC-P: Human kidney, testis, cerebral cortex, and liver tissues; ICC: A549 cells; WB: RT-4 and U-251 MG cell lysates.
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General notes
Previously labelled as TIMM50.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody (ab204486)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TIM50 with ab204486 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-TIM50 antibody (ab204486)All lanes : Anti-TIM50 antibody (ab204486) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Developed using the ECL technique.
Predicted band size: 40 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-TIM50 antibody (ab204486)Immunofluorescent analysis of paraformaldehyde-fixed A549 cells labeling TIM50 using ab204486 at 4 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody (ab204486)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling TIM50 with ab204486 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody (ab204486)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling TIM50 with ab204486 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody (ab204486)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling TIM50 with ab204486 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
