Anti-TIM50 antibody [EPR5784] (ab109527)
![Anti-TIM50 antibody [EPR5784] (ab109527)](https://www.abcam.com/ps/products/109/ab109527/Images/ab109527-122459-anti-tim50-antibody-epr5784-western-blot.jpg "Anti-TIM50 antibody [EPR5784] (ab109527)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5784] to TIM50
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-TIM50 antibody [EPR5784]
See all TIM50 primary antibodies -
Description
Rabbit monoclonal [EPR5784] to TIM50 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- 293T, SH SY5Y, HepG2 and HeLa cell lysates Human kidney tissue, human liver tissue (IHC-P)
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Previously labelled as TIMM50.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5784 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TIM50 antibody [EPR5784] (ab109527)All lanes : Anti-TIM50 antibody [EPR5784] (ab109527) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 40 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody [EPR5784] (ab109527)](https://www.abcam.com/ps/products/109/ab109527/Images/ab109527-122458-anti-tim50-antibody-epr5784-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody [EPR5784] (ab109527)ab109527, at 1/50, staining TIM50 in paraffin-embedded human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody [EPR5784] (ab109527)](https://www.abcam.com/ps/products/109/ab109527/Images/ab109527-122457-anti-tim50-antibody-epr5784-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM50 antibody [EPR5784] (ab109527)ab109527, at 1/50, staining TIM50 in paraffin-embedded human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-TIM50 antibody [EPR5784] (ab109527)](https://www.abcam.com/ps/products/109/ab109527/Images/ab109527-4-benefits-of-recombinant-antibodies.png)
Anti-TIM50 antibody [EPR5784] (ab109527)
