Anti-TIL/TLR1 antibody (ab37068)
Key features and details
- Rabbit polyclonal to TIL/TLR1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TIL/TLR1 antibody
See all TIL/TLR1 primary antibodies -
Description
Rabbit polyclonal to TIL/TLR1 -
Host species
Rabbit -
Specificity
ab37068 recognises Toll-like receptor 1 (TIL/TLR1).
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Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human TIL/TLR1 (C terminal).
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Positive control
- Mouse spleen lysate IF/ICC: RAW246.7 cell line
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified by ion exchange chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TIL/TLR1 antibody (ab37068)Lane 1 : Anti-TIL/TLR1 antibody (ab37068) at 1 µg/ml
Lane 2 : Anti-TIL/TLR1 antibody (ab37068) at 2 µg/ml
Lane 3 : Anti-TIL/TLR1 antibody (ab37068) at 4 µg/ml
All lanes : Mouse spleen lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 90 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIL/TLR1 antibody (ab37068)ab37068 at 10µg/ml staining TIL/TLR1 in mouse spleen tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-TIL/TLR1 antibody (ab37068)ICC/IF image of ab37068 stained RAW246.7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-TIL/TLR1 antibody (ab37068)Immunofluorescence of TIL/TLR1 in Mouse Spleen cells using ab37068 at 20 ug/ml.
