Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815)
![Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815)](https://www.abcam.com/ps/products/214/ab214815/Images/ab214815-313732-anti-tifa-phospho-t9-antibody-epr19853-western-blot.jpg "Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19853] to TIFA (phospho T9)
- Suitable for: WB, Dot blot, IP
- Reacts with: Human
Overview
-
Product name
Anti-TIFA (phospho T9) antibody [EPR19853]
See all TIFA primary antibodies -
Description
Rabbit monoclonal [EPR19853] to TIFA (phospho T9) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB HumanRecombinant fragment
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa transfected with 3×Flag-tagged TIFA expression vector for 24 hours, and then infected with S. flexneri 2457T for 4 hours, whole cell lysate. HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours, whole cell lysate. IP: HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19853 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815)All lanes : Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) transfected with 3×Flag-tagged TIFA expression vector for 28 hours, whole cell lysate
Lane 2 : HeLa transfected with 3×Flag-tagged TIFA T9A mutant expression vector for 28 hours, whole cell lysate
Lane 3 : HeLa transfected with 3×Flag-tagged TIFA expression vector for 24 hours, and then infected with S. flexneri 2457T for 4 hours, whole cell lysate
Lane 4 : HeLa transfected with 3×Flag-tagged TIFAT9A mutant expression vector for 24 hours and then infected with S. flexneri 2457T for 4 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution
Predicted band size: 21 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% milk/TBST.
The image was kindly provided by our collaborator Dr. Feng Shao, NIBS.
S. flexneri infection induces an innate immune response (PMID: 28222186).
-
![Immunoprecipitation - Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815)](https://www.abcam.com/ps/products/214/ab214815/Images/ab214815-313733-anti-tifa-phospho-t9-antibody-epr19853-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815)TIFA (phospho T9) was immunoprecipitated from 0.35 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab214815 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214815 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution
Lane 1: HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours whole cell lysate 10 µg (Input).
Lane 2: ab214815 IP in HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214815 in HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours whole cell lysate (-).Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.The cell lysates were kindly provided by our collaborator Dr. Feng Shao, NIBS.
-
![Western blot - Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815)](https://www.abcam.com/ps/products/214/ab214815/Images/ab214815-313731-anti-tifa-phospho-t9-antibody-epr19853-western-blot.jpg)
Western blot - Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815)All lanes : Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged TIFA expression vector, whole cell lysate
Lane 2 : HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Exposure time: 103 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The band observed represents GFP-tagged TIFA pT9.
The lysates were kindly provided by our collaborator Dr. Feng Shao, NIBS.
B. cenocepacia J2315 infection elicits an inflammatory response. (PMID: 27133449)
-
![Dot Blot - Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815)](https://www.abcam.com/ps/products/214/ab214815/Images/ab214815-313730-anti-tifa-phospho-t9-antibody-epr19853-dot-blot.jpg)
Dot Blot - Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815)Dot blot analysis of TIFA (phospho T9) labeled with ab214815 at 1/1000 dilution.
Lane 1: TIFA (phospho T9) peptide (aa3-12).
Lane 2: TIFA (phospho T9) peptide (aa5-15).
Lane 3: TIFA non-phospho peptide (aa3-15).
Secondary used was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
-
![Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815)](https://www.abcam.com/ps/products/214/ab214815/Images/ab214815-5-benefits-of-recombinant-antibodies.png)
Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815)
