Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free (ab236848)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19853] to TIFA (phospho T9) - BSA and Azide free
  • Suitable for: IP, Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free
    See all TIFA primary antibodies

  • Description

    Rabbit monoclonal [EPR19853] to TIFA (phospho T9) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa transfected with 3×Flag-tagged TIFA expression vector for 24 hours, and then infected with S. flexneri 2457T for 4 hours, whole cell lysate.

  • General notes

    Ab236848 is the carrier-free version of ab214815. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236848 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Western blot - Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free (ab236848)
    Western blot - Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free (ab236848)
    All lanes : Anti-TIFA (phospho T9) antibody [EPR19853] (ab214815) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) transfected with 3×Flag-tagged TIFA expression vector for 28 hours, whole cell lysate
    Lane 2 : HeLa transfected with 3×Flag-tagged TIFA T9A mutant expression vector for 28 hours, whole cell lysate
    Lane 3 : HeLa transfected with 3×Flag-tagged TIFA expression vector for 24 hours, and then infected with S. flexneri 2457T for 4 hours, whole cell lysate
    Lane 4 : HeLa transfected with 3×Flag-tagged TIFAT9A mutant expression vector for 24 hours, and then infected with S. flexneri 2457T for 4 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

    Predicted band size: 21 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% milk/TBST.

    The image was kindly provided by our collaborator Dr. Feng Shao, NIBS.

     S. flexneri infection induces an innate immune response (PMID: 28222186).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab214815).

  • Immunoprecipitation - Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free (ab236848)
    Immunoprecipitation - Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free (ab236848)

    TIFA (phospho T9) was immunoprecipitated from 0.35 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab214815 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214815 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours whole cell lysate 10 µg (Input).
    Lane 2: ab214815 IP in HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214815 in HEK-293T transfected with GFP-tagged TIFA expression vector for 24 hours, and then infected with B. cenocepacia J2315 for 4 hours whole cell lysate (-).

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    The cell lysates were kindly provided by our collaborator Dr. Feng Shao, NIBS.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214815).

  • Dot Blot - Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free (ab236848)
    Dot Blot - Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free (ab236848)

    Dot blot analysis of TIFA (phospho T9) labeled with ab214815 at 1/1000 dilution.

    Lane 1: TIFA (phospho T9) peptide (aa3-12).

    Lane 2: TIFA (phospho T9) peptide (aa5-15).

    Lane 3: TIFA non-phospho peptide (aa3-15).

    Secondary used was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214815).

  • Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free (ab236848)
    Anti-TIFA (phospho T9) antibody [EPR19853] - BSA and Azide free (ab236848)

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