Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23574-405] to Thyroid Peroxidase/TPO - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free
    See all Thyroid Peroxidase/TPO primary antibodies

  • Description

    Rabbit monoclonal [EPR23574-405] to Thyroid Peroxidase/TPO - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat thyroid tissue lysate. IHC-P: Mouse and rat thyroid tissue. IHC-Fr: Mouse and rat thyroid tissue.

  • General notes

    Please Note: Clone [EPR23574-405] (this product) is different to that of ab275932 [EPR23574-130] for the same target.

    ab278531 is the carrier-free version of ab278525. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)
    Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)
    Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525) at 1/1000 dilution + Mouse thyroid tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 103 kDa
    Observed band size: 101 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab278525, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)

    This data was developed using ab278525, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/5000 (0.109 µg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Cytoplasmic staining in mouse thyroid. The section was incubated with ab278525 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)
    Immunohistochemistry (Frozen sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)

    This data was developed using ab278525, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse thyroid tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/500 (1.086 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse thyroid is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)
    Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)
    All lanes : Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] (ab278525) at 1/1000 dilution

    Lane 1 : Rat thyroid tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 103 kDa
    Observed band size: 101 kDa why is the actual band size different from the predicted?



    This data was developed using ab278525, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    TPO is expressed in thyroid. Positive signal is observed in thyroid tissue (PMID: 29541194), no signal is observed in heart tissue.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)

    This data was developed using ab278525, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat thyroid tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/5000 (0.109 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Cytoplasmic staining in rat thyroid. The section was incubated with ab278525 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)
    Immunohistochemistry (Frozen sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)

    This data was developed using ab278525, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat thyroid tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/500 (1.086 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat thyroid is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR23574-405] - BSA and Azide free (ab278531)

    This data was developed using ab278525, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Thyroid Peroxidase/TPO with ab278525 at 1/5000 (0.109 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond®  Polymer Refine Detection) was used. The section was incubated with ab278525 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    No staining in rat cardiac muscle.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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