Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (ab250296)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13203-28] to C8orf30A - BSA and Azide free
  • Suitable for: ICC, WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free
    See all C8orf30A primary antibodies

  • Description

    Rabbit monoclonal [EPR13203-28] to C8orf30A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250296 is the carrier-free version of ab181031. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250296 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13203-28

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (ab250296)
    Western blot - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (ab250296)
    All lanes : Anti-C8orf30A antibody [EPR13203-28] (ab181031) at 1/5000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human spleen tissue lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Caco-2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 42 kDa



    This data was developed using ab181031, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (ab250296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (ab250296)
    This data was developed using ab181031, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human endometrium tissue labeling C8orf30A with ab181031 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (ab250296)
    Immunoprecipitation - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (ab250296)
    This data was developed using ab181031, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (+) or a negative control (-) immunoprecipitated using ab181031 at 1/40 dilution.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
  • Immunocytochemistry - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (ab250296)
    Immunocytochemistry - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (ab250296)
    This data was developed using ab181031, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling C8orf30A with ab181031 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (ab250296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (ab250296)
    This data was developed using ab181031, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling C8orf30A with ab181031 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (ab250296)
    Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free (ab250296)

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