Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Thrombomodulin antibody [EPR18217-209] - BSA and Azide free
See all Thrombomodulin primary antibodies
Description

Rabbit monoclonal [EPR18217-209] to Thrombomodulin - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IHC-Fr	Mouse
IHC-P	Mouse
IP	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Mouse lung tissue.
General notes
Ab230152 is the carrier-free version of ab230010. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab230152 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18217-209
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217-209] - BSA and Azide free (ab230152)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Thrombomodulin with ab230010 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of mouse stomach (PMID: 23946288; PMID: 10231031) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230010).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Thrombomodulin antibody [EPR18217-209] - BSA and Azide free (ab230152)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEND.3 (mouse brain endothelioma cell line) cells labeling Thrombomodulin with ab230010 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in bEND.3 cell line (PMID: 7622601; PMID: 8223719).

The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230010).
Immunoprecipitation - Anti-Thrombomodulin antibody [EPR18217-209] - BSA and Azide free (ab230152)

Thrombomodulin was immunoprecipitated from 0.35 mg of mouse lung tissue lysate with ab230010 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230010 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: Mouse lung tissue lysate 10 μg (Input).
Lane 2: ab230010 IP in mouse lung tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230010 in mouse lung tissue lysate.

Exposure time: 10 seconds.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230010).
Immunoprecipitation - Anti-Thrombomodulin antibody [EPR18217-209] - BSA and Azide free (ab230152)

Thrombomodulin was immunoprecipitated from 0.35 mg of bEND.3 (mouse brain endothelioma cell line) whole cell lysate with ab230010 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230010 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: bEND.3 whole cell lysate 10 μg (Input).
Lane 2: ab230010 IP in bEND.3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230010 in bEND.3 whole cell lysate.

Exposure time: 10 seconds.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230010).
Flow Cytometry - Anti-Thrombomodulin antibody [EPR18217-209] - BSA and Azide free (ab230152)

Flow cytometric analysis of bEND.3 (mouse brain endothelioma cell line) cells labeling Thrombomodulin with ab230010 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

Gated on total viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230010).
Immunohistochemistry (Frozen sections) - Anti-Thrombomodulin antibody [EPR18217-209] - BSA and Azide free (ab230152)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse embryo E14.5 (developing lung) tissue labeling Thrombomodulin with ab230010 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive membrane staining in the developing lung in mouse E14.5 embryo (PMID: 28306049) is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230010).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217-209] - BSA and Azide free (ab230152)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Thrombomodulin with ab230010 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of mouse lung (PMID: 23946288; PMID: 10231031) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230010).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Thrombomodulin antibody [EPR18217-209] - BSA and Azide free (ab230152)