Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free (ab239919)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [51-8] to Thiophosphate ester - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Species independent
Overview
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Product name
Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free
See all Thiophosphate ester primary antibodies -
Description
Rabbit monoclonal [51-8] to Thiophosphate ester - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Thiophosphate ester conjugated to keyhole limpet haemocyanin.
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General notes
ab239919 is the carrier-free version of ab92570 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab239919 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Kevan Shokat at University of California at San Francisco. It is possible to use ab92570 with ab138911 as a kinase reaction reagent for the identification of direct kinase substrates as mentioned in Allen JJ et al. Nat Methods 4:511-6 (2007).After the kinase of interest has accepted ATP-γ-S (ab138911), p -Nitrobenzyl mesylate (ab138910) can be used to alkylate the thiophospholyation site on the substrates. ab92570 is introduced to identify the tagged substrates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
51-8 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Thiophosphate ester antibody [51-8] (ab92570) at 1/5000 dilution
Lane 1 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug)
Lane 2 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using alkylation with PNBM (2.5mM).
Lane 3 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S
Lane 4 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S followed by alkylation with PNBM (2.5mM).This data was developed using ab92570, the same antibody clone in a different buffer formulation.
In vitro kinase reaction was carried out for 30 minutes at room temperature. PNBM alkylation (2.5mM) was for 2 hours at room temperature. Reaction products were analyzed by western blot with thiophosphate ester specific ab92570 at 1/5000, alpha-GST antibody confirms equal loading.
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