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Cell Biology Other Antibodies Drugs and Chemicals

Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free (ab239919)

Price and availability

526 012 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free (ab239919)
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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [51-8] to Thiophosphate ester - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Species independent

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Overview

  • Product name

    Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free
    See all Thiophosphate ester primary antibodies
  • Description

    Rabbit monoclonal [51-8] to Thiophosphate ester - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Thiophosphate ester conjugated to keyhole limpet haemocyanin.

  • General notes

    ab239919 is the carrier-free version of ab92570 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239919 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Kevan Shokat at University of California at San Francisco. It is possible to use ab92570 with ab138911 as a kinase reaction reagent for the identification of direct kinase substrates as mentioned in Allen JJ et al. Nat Methods 4:511-6 (2007).After the kinase of interest has accepted ATP-γ-S (ab138911), p -Nitrobenzyl mesylate (ab138910) can be used to alkylate the thiophospholyation site on the substrates. ab92570 is introduced to identify the tagged substrates.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    51-8
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Drugs and Chemicals

Images

  • Western blot - Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free (ab239919)
    Western blot - Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free (ab239919)
    All lanes : Anti-Thiophosphate ester antibody [51-8] (ab92570) at 1/5000 dilution

    Lane 1 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug)
    Lane 2 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using alkylation with PNBM (2.5mM).
    Lane 3 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S
    Lane 4 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S followed by alkylation with PNBM (2.5mM).


    This data was developed using ab92570, the same antibody clone in a different buffer formulation.

    In vitro kinase reaction was carried out for 30 minutes at room temperature. PNBM alkylation (2.5mM) was for 2 hours at room temperature. Reaction products were analyzed by western blot with thiophosphate ester specific ab92570 at 1/5000, alpha-GST antibody confirms equal loading.

  • Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free (ab239919)
    Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free (ab239919)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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