Anti-TAMRA antibody [5G5] (ab171120)
Key features and details
- Mouse monoclonal [5G5] to TAMRA
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-TAMRA antibody [5G5]
See all TAMRA primary antibodies -
Description
Mouse monoclonal [5G5] to TAMRA -
Host species
Mouse -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to TAMRA.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5G5 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation was performed on purified TAMRA-BSA. The antigen:antibody complex was formed by binding 5 µg protein with 10 µg of ab171120 or mouse IgG, whole molecule overnight at 4°C. The immune-complex was then captured on 50 µl Protein A/G Plus Agarose. Captured immune-complexes were then washes extensively and proteins eluted with sample loading dye. Samples were resolved on a SDS PAGE gel.
Lane 1: ab171120
Lane 2: BSA
Lane 3: TAMRA-BSA
Lane 4: BSA-ab171120
Lane 5: TAMRA-BSA + mouse IgG
Lane 6: TAMRA-BSA + ab171120
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All lanes : Anti-TAMRA antibody [5G5] (ab171120) at 1/1000 dilution
Lane 1 : TAMRA-BSA at 10 µg
Lane 2 : TAMRA-BSA at 5 µg
Lane 3 : TAMRA-BSA at 2 µg
Lane 4 : TAMRA-BSA at 1 µg
Lane 5 : TAMRA-BSA at 0.5 µg
Lane 6 : TAMRA-BSA at 0 µg
Secondary
All lanes : Goat anti-mouse-HRP
Developed using the ECL technique.