Anti-TGN46 antibody (ab50595)
Key features and details
- Rabbit polyclonal to TGN46
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TGN46 antibody
See all TGN46 primary antibodies -
Description
Rabbit polyclonal to TGN46 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide:
KASDYQRLDQKY
conjugated to KLH by a N-terminal Cysteine residue linker, corresponding to amino acids 426-437 of Human TGN46 -
Positive control
- A549 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595) This image is courtesy of an abreview submitted by Dr Armen Petrosyan, Univ of Nebraska.
Immunocytochemistry/ Immunofluorescence analysis of Panc-1 cells labeling TGN46 with ab50595 at 1/100 dilution. Cells were fixed with formaldehyde followed by blocking in 1% Donkey serum in PBST for 1 hour at 22°C. A donkey-anti-Rb Alexa Fluor® 594 was used as the secondary antibody at 1/200 dilution. DAPI nulcear counter stain.
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Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595) This image is courtesy of an anonymous Abreviewab50595 staining TGN46 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde in PBS for 10 minutes, permeabilized with 0.1% Triton X100 and blocked with 2% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 2% BSA + 0.1% Triton X100) for 2 hours at 25°C. An Alexa Fluor® 594-conjugated Goat polyclonal to mouse IgG (dilution 1/1500) was used as secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595)Human A549 cells were fixed and permeabilized with methanol followed by acetone fixation. Fixed cells were stained with 2.5 µg/ml ab50595. The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate. -
Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595) This image is courtesy of Alessandro Marcello, Int Ctr Genet Engn & Biotechnol, Italyab50595 staining human osteosarcoma (143b) cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor® 594 conjugated donkey polyclonal to rabbit diluted 1/500.
