Anti-TGN46 antibody - Golgi Marker (ab16059)
Key features and details
- Rabbit polyclonal to TGN46 - Golgi Marker
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-TGN46 antibody - Golgi Marker
See all TGN46 primary antibodies -
Description
Rabbit polyclonal to TGN46 - Golgi Marker -
Host species
Rabbit -
Specificity
This antibody does not cross react with TGN46 - the human homologue of TGN46. A Rabbit polyclonal to human TGN46 is available (ab16052). -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Chinese hamster
Does not react with:
Human -
Immunogen
Synthetic peptide corresponding to Mouse TGN46 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab16384)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab16059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ICC Use a concentration of 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 35 kDa). Rodent TGN46 is extensively glycosylated and as a result has a mass of 97 kDa on an SDS-PAGE gel.
Target
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Function
May be involved in regulating membrane traffic to and from trans-Golgi network. -
Tissue specificity
Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells. -
Cellular localization
Cell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes. - Information by UniProt
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Database links
- Entrez Gene: 22135 Mouse
- Entrez Gene: 192152 Rat
- SwissProt: Q62314 Mouse
- Unigene: 246563 Mouse
- Unigene: 11349 Rat
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Alternative names
- TGN 46 antibody
- TGN 51 antibody
- TGN38 antibody
see all
Images
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Immunocytochemistry - Anti-TGN46 antibody - Golgi Marker (ab16059)
ICC/IF image of ab16059 stained mouse MEF1 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab16059, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Western blot - Anti-TGN46 antibody - Golgi Marker (ab16059)All lanes : Anti-TGN46 antibody - Golgi Marker (ab16059) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.Rodent TGN46 is extensively glycosylated and as a result has a mass of 97 kDa on an SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody - Golgi Marker (ab16059)IHC image of ab16059 staining in mouse e14 whole foetus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16059, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry - Anti-TGN46 antibody - Golgi Marker (ab16059)Paraformaldehyde-fixed, Triton-X 100-permeabilised mouse keratinocytes stained for TGN46 at 1/200 dilution in ICC.
Protocols
Datasheets and documents
References (26)
ab16059 has been referenced in 26 publications.
- Budzinska MI et al. PTPN23 binds the dynein adaptor BICD1 and is required for endocytic sorting of neurotrophin receptors. J Cell Sci 133:N/A (2020). PubMed: 32079660
- Shitara A et al. Cdc42 negatively regulates endocytosis during apical membrane maintenance in live animals. Mol Biol Cell 30:324-332 (2019). PubMed: 30540520
- Han F et al. Globozoospermia and lack of acrosome formation in GM130-deficient mice. Cell Death Dis 8:e2532 (2017). PubMed: 28055014
- Kage F et al. FMNL2 and -3 regulate Golgi architecture and anterograde transport downstream of Cdc42. Sci Rep 7:9791 (2017). PubMed: 28852060
- Godbole A et al. Internalized TSH receptors en route to the TGN induce local Gs-protein signaling and gene transcription. Nat Commun 8:443 (2017). PubMed: 28874659
Images
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Immunocytochemistry - Anti-TGN46 antibody - Golgi Marker (ab16059)
ICC/IF image of ab16059 stained mouse MEF1 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab16059, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Western blot - Anti-TGN46 antibody - Golgi Marker (ab16059)All lanes : Anti-TGN46 antibody - Golgi Marker (ab16059) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.Rodent TGN46 is extensively glycosylated and as a result has a mass of 97 kDa on an SDS-PAGE gel.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody - Golgi Marker (ab16059)IHC image of ab16059 staining in mouse e14 whole foetus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16059, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry - Anti-TGN46 antibody - Golgi Marker (ab16059)
Paraformaldehyde-fixed, Triton-X 100-permeabilised mouse keratinocytes stained for TGN46 at 1/200 dilution in ICC.
