Human P-Selectin ELISA Kit (CD62P) (ab272202)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 89 pg/ml
- Range: 100 pg/ml - 6400 pg/ml
- Sample type: Cell culture media, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human P-Selectin ELISA Kit (CD62P)
See all CD62P kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.5% Inter-assay Sample n Mean SD CV% Serum 4 2.7% -
Sample type
Serum, Cell culture media, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
89 pg/ml -
Range
100 pg/ml - 6400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 116 114% - 121% Cell culture media 87 81% - 91% EDTA Plasma 98 87% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human P-Selectin ELISA kit (ab272200) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of P-Selectin protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human P-Selectin with 89 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
P-selectin, also known as CD62, Granule membrane protein 140, Leukocyte-endothelial cell adhesion molecule 3, or platelet activation dependent granule-external membrane protein, is a cell adhesion molecule (CAM) encoded by the SELP gene. P-selectin is Ca2+ dependent cell-surface glycoprotein that functions in the migration of lymphocytes into tissues during inflammation. P-selectin expressed by megakaryocytes and endothelial cells, where it is stored in Weibel-Palade bodies and alpha-granules. In the presence of agonists such as thrombin, P-selectin is translocated to the plasma membrane from granules. P-selectin expression is also induced by inflammatory agents such as; TNF-a, LPS, and IL-4. The ligand for P-selectin is P-Selectin glycoprotein ligand-1 (PSGL-1), which is primarily expressed on all leukocytes.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human P-Selectin (CD62P) Capture Antibody 1 x 600µl 10X Human P-Selectin (CD62P) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human P-Selectin (CD62P) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. -
Tissue specificity
Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. -
Involvement in disease
Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 9 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Antigen CD62
- CD62
- CD62 antigen-like family member P
see all -
Database links
- Entrez Gene: 6403 Human
- Omim: 173610 Human
- SwissProt: P16109 Human
- Unigene: 73800 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human P-Selectin competitor curve comparisonStandard Curve comparison between Human P-Selectin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human P-Selectin standard curve in Sample Diluent NS.The P-Selectin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native P-Selectin in human serum and plasma (EDTA).The concentrations of P-Selectin were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 10% and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 65.6 ng/mL in serum and 54.3 ng/mL in plasma (EDTA).
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Serum from eight individual healthy P-Selectin gender donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean P-Selectin concentration was determined to be 76.7 ng/mL with a range of 50.1 – 163.8 ng/mL.
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Sandwich ELISA - Human P-Selectin ELISA Kit (CD62P) (ab272202)To learn more about the advantages of recombinant antibodies see here.
