Recombinant human COX2 / Cyclooxygenase 2 protein (ab198646)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His-DDDDK tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human COX2 / Cyclooxygenase 2 protein
See all COX2 / Cyclooxygenase 2 proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MLARALLLCAVLALSHTANPCCSHPCQNRGVCMSVGFDQYKCDCTRTGFY GENCSTPEFLTRIKLFLKPTPNTVHYILTHFKGFWNVVNNIPFLRNAIMS YVLTSRSHLIDSPPTYNADYGYKSWEAFSNLSYYTRALPPVPDDCPTPLG VKGKKQLPDSNEIVEKLLLRRKFIPDPQGSNMMFAFFAQHFTHQFFKTDH KRGPAFTNGLGHGVDLNHIYGETLARQRKLRLFKDGKMKYQIIDGEMYPP TVKDTQAEMIYPPQVPEHLRFAVGQEVFGLVPGLMMYATIWLREHNRVCD VLKQEHPEWGDEQLFQTSRLILIGETIKIVIEDYVQHLSGYHFKLKFDPE LLFNKQFQYQNRIAAEFNTLYHWHPLLPDTFQIHDQKYNYQQFIYNNSIL LEHGITQFVESFTRQIAGRVAGGRNVPPAVQKVSQASIDQSRQMKYQSFN EYRKRFMLKPYESFEELTGEKEMSAELEALYGDIDAVELYPALLVEKPRP DAIFGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASI QSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKER STELDYKDDDDKHHHHHH -
Predicted molecular weight
71 kDa including tags -
Amino acids
1 to 604 -
Tags
His-DDDDK tag C-Terminus -
Additional sequence information
GenBank Accession No. NM_000963
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.08% Triton X-100, 0.01% Diethyldithiocarbamate
Contains 80 µg/ml DDDDK peptide.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant human COX2 / Cyclooxygenase 2 protein (ab198646)
4-20% SDS Page analysis of ab198646 (2 μg)
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Functional Studies - Recombinant human COX2 / Cyclooxygenase 2 protein (ab198646)Mixed various concentrations of enzyme in assay buffer (100 mM Tris-HCl pH 8, 3 uM EDTA) with 5 μM Hematin for 10 min then added 100 M Ampliflu Red and 200 μM arachidonic acid. Fluorescence (ex. 535 em. 590) was measured after 5 minutes at room temperature. Reaction volume was 100 μl.
