Anti-TFIIF antibody (ab28180)
Key features and details
- Rabbit polyclonal to TFIIF
- Suitable for: ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TFIIF antibody
See all TFIIF primary antibodies -
Description
Rabbit polyclonal to TFIIF -
Host species
Rabbit -
Specificity
This antibody is specific to the RAP30 subunit of TFIIF. -
Tested Applications & Species
See all applications and species dataApplication Species ChIP Human
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Immunogen
Recombinant full length protein - RAP30 subunit of the TFIIF complex.
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Positive control
- HeLa cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.9
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-TFIIF antibody (ab28180)
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 25µg of chromatin, 8µg of ab28180 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.
