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Epigenetics and Nuclear Signaling Transcription Polymerase associated factors Pol II Transcription

Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free (ab270284)

Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free (ab270284)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [CTD4H8] to RNA polymerase II CTD repeat YSPTSPS - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free
    See all RNA polymerase II CTD repeat YSPTSPS primary antibodies
  • Description

    Mouse monoclonal [CTD4H8] to RNA polymerase II CTD repeat YSPTSPS - BSA and Azide free
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to RNA polymerase II CTD repeat YSPTSPS. Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5.
    Database link: P24928

  • Positive control

    • IHC-P: Human breast carcinoma tissue. WB: HAP1, K562, PC3, HepG2 and NIH/3T3 lysate.
  • General notes

    ab270284 is a carrier free version of ab270250.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from bioreactor concentrate.
  • Clonality

    Monoclonal
  • Clone number

    CTD4H8
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Polymerase associated factors
    • Pol II Transcription
    • Polymerase
    • Epigenetics and Nuclear Signaling
    • Transcription
    • RNA polymerase

Images

  • Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free (ab270284)
    Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free (ab270284)
    All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] (ab270250) at 2 µg/ml

    Lane 1 : HAP1 (Human cell line) lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) lysate
    Lane 3 : PC3 (Human prostate adenocarcinoma cell line) lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) lysate
    Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) lysate


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270250).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free (ab270284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free (ab270284)

    Formalin-fixed, paraffin-embedded human breast carcinoma stained for RNA polymerase II CTD repeat YSPTSPS using ab270250 at 2 µg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270250).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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