Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free (ab270284)
![Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free (ab270284)](https://www.abcam.com/ps/products/270/ab270284/Images/ab270284-381136-mouse-monoclonal-antibody-ctd4h8-wb-hap1-k562-pc3-hepg2--nih3t3-cell-lysates.jpg "Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free (ab270284)")
Key features and details
- Mouse monoclonal [CTD4H8] to RNA polymerase II CTD repeat YSPTSPS - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
-
Product name
Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free
See all RNA polymerase II CTD repeat YSPTSPS primary antibodies -
Description
Mouse monoclonal [CTD4H8] to RNA polymerase II CTD repeat YSPTSPS - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman
-
Immunogen
Synthetic peptide corresponding to RNA polymerase II CTD repeat YSPTSPS. Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5.
Database link: P24928 -
Positive control
- IHC-P: Human breast carcinoma tissue. WB: HAP1, K562, PC3, HepG2 and NIH/3T3 lysate.
-
General notes
ab270284 is a carrier free version of ab270250.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
CTD4H8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free (ab270284)All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] (ab270250) at 2 µg/ml
Lane 1 : HAP1 (Human cell line) lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) lysate
Lane 3 : PC3 (Human prostate adenocarcinoma cell line) lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) lysateThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270250).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free (ab270284)](https://www.abcam.com/ps/products/270/ab270284/Images/ab270284-381132-mouse-monoclonal-antibody-ctd4h8-ihcp-breast-carcinoma-tissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA polymerase II CTD repeat YSPTSPS antibody [CTD4H8] - BSA and Azide free (ab270284)Formalin-fixed, paraffin-embedded human breast carcinoma stained for RNA polymerase II CTD repeat YSPTSPS using ab270250 at 2 µg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270250).