Anti-TEAD4 antibody (ab151274)
Key features and details
- Rabbit polyclonal to TEAD4
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TEAD4 antibody
See all TEAD4 primary antibodies -
Description
Rabbit polyclonal to TEAD4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human TEAD4 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab151396) -
Positive control
- Purchase matching WB positive control:Recombinant Human TEAD4 protein
- This antibody gave a positive signal in the following whole cell lysates: HepG2; HuES7; JEG3; SW480; Caco2; HCT116.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC HumanWB HumanAll applications RabbitHorseCowDogPigChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).Target
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Function
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif. -
Tissue specificity
Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart. -
Sequence similarities
Contains 1 TEA DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7004 Human
- Entrez Gene: 100152085 Pig
- Omim: 601714 Human
- SwissProt: Q15561 Human
- Unigene: 94865 Human
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Alternative names
- EFTR 2 antibody
- EFTR2 antibody
- hRTEF 1B antibody
see all
Images
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Western blot - Anti-TEAD4 antibody (ab151274)All lanes : Anti-TEAD4 antibody (ab151274) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TEAD4 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 48 kDaLanes 1 - 4: Merged signal (red and green). Green - ab151274 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab151274 was shown to recognize TEAD4 in wild-type HAP1 cells as signal was lost at the expected MW in TEAD4 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TEAD4 knockout samples were subjected to SDS-PAGE. Ab151274 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-TEAD4 antibody (ab151274)All lanes : Anti-TEAD4 antibody (ab151274) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 6 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 40 kDa, 66 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab151274 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151274 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-TEAD4 antibody (ab151274)All lanes : Anti-TEAD4 antibody (ab151274) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TEAD4 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 48 kDaLanes 1 - 4: Merged signal (red and green). Green - ab151274 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab151274 was shown to recognize TEAD4 in wild-type HAP1 cells as signal was lost at the expected MW in TEAD4 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TEAD4 knockout samples were subjected to SDS-PAGE. Ab151274 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-TEAD4 antibody (ab151274)All lanes : Anti-TEAD4 antibody (ab151274) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 6 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa, 40 kDa, 66 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab151274 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
